tag:blogger.com,1999:blog-8037707352330428788.post3694843600558247420..comments2024-01-26T04:59:37.784-06:00Comments on UCFlow - Flow Cytometry news, reviews, and tips.: Compensation is infiltrating my dreams.UC Flowhttp://www.blogger.com/profile/03187449850452376466noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-8037707352330428788.post-2675629274438248732010-12-17T15:08:54.542-06:002010-12-17T15:08:54.542-06:00I usually use the beads at about 1/10th the indica...I usually use the beads at about 1/10th the indicated concentration, bringing the price down 10 fold.<br />Never thought of using 2 different ab concentrations instead of the negative beads though... will try it.Cesarnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-56561769119802929982010-11-11T08:44:48.543-06:002010-11-11T08:44:48.543-06:00I do see what you're saying, and I agree; it w...I do see what you're saying, and I agree; it would be nice if our researchers could bring us always bead compensation controls. However, many researchers dislike change, and beads are expensive; the "I hate change" camp would also disapprove of thymus controls. Then again these are often the same people who use FITC as a control for GFP or A488.<br /><br />We do rely on cell controls, and request users that are compensating with beads bring cell ones as well. Our main reason is to ensure the staining worked, and is staining the appropriate cells. How many times has Joe Schmo brought you CDblaty-blah bone marrow, and then found its beautiful stain is only on dead cells? :)Laurahttps://www.blogger.com/profile/05032423174545434167noreply@blogger.com