tag:blogger.com,1999:blog-8037707352330428788.post56921278322998686..comments2024-01-26T04:59:37.784-06:00Comments on UCFlow - Flow Cytometry news, reviews, and tips.: ISAC Wrap-up Part 1UC Flowhttp://www.blogger.com/profile/03187449850452376466noreply@blogger.comBlogger27125tag:blogger.com,1999:blog-8037707352330428788.post-36721207570659493072021-01-13T03:56:44.204-06:002021-01-13T03:56:44.204-06:00I impressed by the quality of information on this ...I impressed by the quality of information on this website. There are a lot of good resources here. I am sure I will visit this place again soon. <a href="https://invictussolutions.co/" rel="nofollow">software house in faisalabad</a><br />Rice Storehttps://www.blogger.com/profile/05493113109799507218noreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-83227510988335473372008-11-08T02:50:00.000-06:002008-11-08T02:50:00.000-06:00I know the request was for Mac software but someti...I know the request was for Mac software but sometimes we have to <BR/>swallow<BR/><BR/>From: David Novo (no...@fhs.csu.McMaster.CA) <BR/>Date: Thu Feb 26 1998 - 17:00:40 EST <BR/>• Next message: JoAnne Thomas: "FACSLoader Upgrade" <BR/>• Previous message: David McFarland: "MO drives and CellQuest <BR/>files" <BR/>• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>If I am not mistaken, WinMDI comes with a little utility called <BR/>FCSread <BR/>that extracts file header information. <BR/><BR/><BR/>I know the request was for Mac software but sometimes we have to <BR/>swallow <BR/>our pride and admit we live in Bill's universe :-( <BR/><BR/><BR/>-Dave <BR/>______________________________________... <BR/>• Next message: JoAnne Thomas: "FACSLoader Upgrade" <BR/>• Previous message: David McFarland: "MO drives and CellQuest <BR/>files" <BR/>• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - <BR/>17:35:13 EST <BR/><BR/><BR/>Wanted- FCS keyword read ut <BR/>From: Diether (diet...@amcell.com) <BR/>Date: Tue Feb 24 1998 - 07:57:50 EST <BR/>• Next message: Marty_Gied...@cc.chiron.com: "Annexin V <BR/>expression" <BR/>• Previous message: Colleen Hou-En Woo: "FDG and cell surface <BR/>markers" <BR/>• Next in thread: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Reply: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>Subject: Time: <BR/>13:54 <BR/>OFFICE MEMO Wanted: FCS keyword read utility for Date: <BR/>2/24/98 <BR/>I am looking for a utility program for the MacIntosh to read and <BR/>print <BR/>filenames <BR/>and FCS keywords like $BTIM and $ETIM. <BR/><BR/><BR/>Diether R. <BR/>AmCell Corp. <BR/>Ph.: USA-408-752-1200 <BR/>FAX: USA-408-752-1212 <BR/>Email: diet...@amcell.com or for binary attachments diethe...@usa.net <BR/>______________________________________... <BR/>• Next message: Marty_Gied...@cc.chiron.com: "Annexin V <BR/>expression" <BR/>• Previous message: Colleen Hou-En Woo: "FDG and cell surface <BR/>markers" <BR/>• Next in thread: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Reply: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - <BR/>17:35:13 EST <BR/><BR/><BR/>Re: Wanted- FCS keyword read ut <BR/>From: David Novo (no...@fhs.csu.McMaster.CA) <BR/>Date: Thu Feb 26 1998 - 17:00:40 EST <BR/>• Next message: JoAnne Thomas: "FACSLoader Upgrade" <BR/>• Previous message: David McFarland: "MO drives and CellQuest <BR/>files" <BR/>• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>If I am not mistaken, WinMDI comes with a little utility called <BR/>FCSread <BR/>that extracts file header information. <BR/><BR/><BR/>I know the request was for Mac software but sometimes we have to <BR/>swallow <BR/>our pride and admit we live in Bill's universe :-( <BR/><BR/><BR/>-Dave <BR/>______________________________________... <BR/>• Next message: JoAnne Thomas: "FACSLoader Upgrade" <BR/>• Previous message: David McFarland: "MO drives and CellQuest <BR/>files" <BR/>• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - <BR/>17:35:13 EST <BR/><BR/><BR/>Re: Wanted- FCS keyword read ut <BR/>From: Ray Hicks (rh...@cus.cam.ac.uk) <BR/>Date: Thu Feb 26 1998 - 05:10:28 EST <BR/>• Next message: Steve G. Hilliard: "For Palm Pilot users" <BR/>• Previous message: Howard Shapiro: "Re: PY staining" <BR/>• In reply to: Diether: "Wanted- FCS keyword read ut" <BR/>• Next in thread: David Novo: "Re: Wanted- FCS keyword read ut" <BR/>• Reply: David Novo: "Re: Wanted- FCS keyword read ut" <BR/>• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>______________________________________... <BR/>Hi Diether, <BR/><BR/><BR/>If you can't find one, try opening the files in microsoft Word <BR/>(you'll <BR/>probably need to use the "All Files" option),copy the text part to a <BR/>new <BR/>window and then get it to replace the delimiter with a paragraph <BR/>mark. <BR/>This <BR/>will give you a list that looks like this: <BR/><BR/><BR/>$TOT <BR/>5000 <BR/>$P1R <BR/>1024 <BR/>$P1B <BR/>16 <BR/>$P2R <BR/>1024 <BR/>$P2B <BR/>16 <BR/>$P3R <BR/>1024 <BR/>$P3B <BR/>16 <BR/>$P4R <BR/>1024 <BR/>$P4B [snip] <BR/><BR/><BR/>Rather than this: <BR/><BR/><BR/>\$TOT\5000\$P1R\1024\$P1B\16\$P2R\1024... <BR/>\1024\$P4B\Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-58517777737849389282008-10-28T13:22:00.000-05:002008-10-28T13:22:00.000-05:00Purdue Cytometry Mailing List: SV: Thanks for the ...Purdue Cytometry Mailing List: SV: Thanks for the suggestions - <BR/><BR/><BR/>... cytometry/WEASELv2.html<BR/><BR/>http://www.wehi.edu.au/cytometry/WEASELv2.html <BR/><BR/><BR/>Winlist 3d from Verity House Software (www.vsh.com) Regards Tim <BR/><BR/><BR/>Timothy Bushnell, Ph.D. Director, CPBR ... Contemporary messages <BR/>sorted: <BR/><BR/>[ By Date ] [ By Thread ] [ By Subject ] [ By Author ... <BR/>http://www.cyto.purdue.edu/hmarchiv/2006/0914.htm - 7.0KB <BR/>70% <BR/>|||||||||||||||||||| <BR/>16 May 06 <BR/>Find Similar <BR/>Highlight <BR/>Re: Thanks for the suggestions - was rendering in 3D<BR/><BR/>This message: [ Message body ] [ More options ]<BR/>Related messages: [ Next message ] [ Previous message ] [ In reply<BR/>to ] [ Next in thread ] [ Replies ]<BR/>From: Adam Treister <...@treestar.com><BR/>Date: Thu May 18 2006 - 20:20:18 EDT<BR/><BR/>On May 12, 2006, at 10:02 AM, Bushnell, Timothy wrote:<BR/><BR/>> Thanks to everyone who suggested possible software to view data in<BR/>> 3D. I’ll be trying several different platforms to see which works<BR/>> best for our applications.<BR/>><BR/><BR/><BR/>> The suggested platforms include (in alphabetical order):<BR/>><BR/><BR/><BR/>> Coulters CXP software for the FC500<BR/>><BR/>> Rflowcyt<BR/>><BR/><BR/><BR/><BR/>> Weasel: http://www.wehi.edu.au/cytometry/WEASELv2.html<BR/>><BR/><BR/><BR/>> Winlist 3d from Verity House Software (www.vsh.com)<BR/>><BR/>> Regards<BR/>><BR/>> TimTim,<BR/><BR/><BR/><BR/>With all due respect to these solutions, you shouldn't think that<BR/><BR/><BR/><BR/>Mario could sleep at night <BR/><BR/><BR/><BR/>if anyone could perform high dimensional<BR/>analysis better than FlowJo. <BR/><BR/><BR/><BR/>[Disclaimer: Yes, I live off FlowJo<BR/>sales, and that's a blatantly commercial statement, but it gets<BR/>technical from here on.]<BR/><BR/><BR/><BR/>We've played with spatial 3D plots a lot, both our own prototypes<BR/>and<BR/>others, and they just don't do a very good job of discriminating<BR/>populations. And its impossible to coherently describe the<BR/>populations you can see.<BR/><BR/> I like the slice-and-dice approach that<BR/>you get by making a flipbook on X vs. Y in slices along the Z axis<BR/>(it shows up as a Quicktime movie), but that too is very difficult<BR/>to<BR/>use in a way that's better than two 2D graphs connected by a gate.<BR/>If you really want to increase your dimensionality, <BR/><BR/><BR/>we're just adding a new "Polyvariate Plot" to the Mac version of FlowJo for next<BR/>week's<BR/><BR/>ISAC. <BR/><BR/>The idea was taken from RFlowCyt. We've added interface<BR/>refinements to make it more interactive, but like any good R tool,<BR/>it'll astound and confuse you.<BR/>http://www.flowjo.com/v8/html/polyvarplot.html<BR/><BR/>The Polyvariate Plot can model transformations in any number of<BR/>dimensions. So it will produce a 3D plot, or as many dimensions as<BR/>you want (Shown below in 5D). And it projects these transformations<BR/>onto a graph window, so you can gate on them.<BR/><BR/>We're still trying to figure out the applications for this<BR/>visualization, but if you're looking for another dimension as a way<BR/>to differentiate populations, we think this is potentially much more<BR/>powerful than conventional spatial projections. This is explained<BR/>in the poster P178 at ISAC next week, or at the web page above.<BR/><BR/>Be forewarned: This is the opposite end of the sizzle/steak<BR/>spectrum. Most people use 3D graphs to make their PowerPoints look<BR/>spiffy. These graphs are absolutely impossible to explain in a<BR/>presentation.<BR/><BR/>Adam<BR/><BR/>-----------------------<BR/><BR/>A 3D plot:<BR/><BR/><BR/><BR/>A 5D plot:<BR/><BR/><BR/>Received on Fri May 19 17:58:00 2006<BR/>This message: [ Message body ]<BR/>Next message: Jerry Spangrude: "Making tandem conjugates"<BR/>Previous message: Rebe...@UCHSC.edu: "Sorting dendritic cells<BR/>and platelets on FACSAria"<BR/>In reply to: Bushnell, <BR/><BR/>Timothy: "Thanks for the suggestions - was<BR/>rendering in 3D"<BR/><BR/><BR/>Next in thread: Jerry Spangrude: "Making tandem conjugates"<BR/>Reply: Jerry Spangrude: "Making tandem conjugates"<BR/>Reply: A.J. Rossini: "Re: Thanks for the suggestions - was rendering<BR/>in 3D"<BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ]<BR/>[ By Author ] [ By messages with attachments ]<BR/>This archive was generated by hypermail 2.1.8 : Wed May 24 2006 -<BR/>04:12:03 EDT<BR/><BR/>This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - <BR/>17:35:29 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-83496397186002483092008-10-26T00:07:00.000-05:002008-10-26T00:07:00.000-05:00If anyone is interested in their software to funct...If anyone is interested in their software to function properly please <BR/>contact Kanecki Associates Inc. <BR/><BR/>In November of 2007 Kanecki Associates Inc developed the FCS Cytopro <BR/>to resolve the Memory issues with the Diva Software after a request <BR/>from one person at BD. 10 Million Events By 40 Parameters with all <BR/>128 <BR/>Permeations. <BR/><BR/><BR/>As of today’s posts on the Purdue Cytometry Mail List the issues <BR/>still <BR/>exist. <BR/><BR/><BR/>Since are not allowed on the Purdue Cytometry Mail List and have ISAC <BR/>Congress Executives blocking us from the market we would like to make <BR/>BD and all other Corporations, Hospitals, Universities, etc. aware <BR/>that we can LICENSE A PATCH that will resolve the issues in BD's DIVA <BR/>software. <BR/><BR/><BR/>We are willing to work with BD and are unaware if they knew of the <BR/>one <BR/>person’s prior request or even what was done with the software once <BR/>the one person from BD received it. We will send a letter to BD's <BR/>corporate office to notify them we are willing to work with the <BR/>corporation if they wish. We did develop the code and are willing to <BR/>work directly with BD if they wish to compile it to the troubled <BR/>software to end all the issues. <BR/><BR/><BR/>There is no need for Purdue University Cytometry Laboratories <BR/>Executives to keep up with suspected exclusion of those who can help. <BR/>They need to resolve the issues in the system with the ISAC Congress <BR/>Executives in the Data Standards Committee and work with other <BR/>corporations even if the won't benefit financially personally. <BR/><BR/><BR/>If anyone is interested in their software to function properly please <BR/>contact Kanecki Associates Inc. <BR/><BR/><BR/>Http://www.kanecki.com <BR/>C...@Kanecki2.com <BR/>Mi...@kanecki.com <BR/><BR/><BR/>Mitchell Haynes <BR/>Vp. <BR/>Kanecki Associates Inc <BR/>281-444-1619 <BR/><BR/><BR/>Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ Next in <BR/>thread ] [ Replies ] <BR/>From: Wayne Turnbull wayne.turnb...@kcl.ac.uk <BR/>Date: Tue Oct 14 2008 - 09:32:19 EDT <BR/><BR/><BR/>Afternoon flowers, <BR/><BR/><BR/>Having recently moved to Diva 6.1.1, we're experiencing some software <BR/>lock-up problems, usually after closing down CS&T, that require a <BR/>full re-install, which is becoming tiresome, and I wondered if any <BR/>others had experienced similar problems? <BR/><BR/><BR/>Wayne <BR/><BR/><BR/>Wayne Turnbull <BR/>Flow Cytometry Services <BR/>Department of Immunobiology <BR/>GKT Guy's Hospital <BR/>London SE1 9RT <BR/>Received on Tue Oct 14 11:58:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Maria Godinho Duarte Soares: "Brdu Labelling" <BR/>Previous message: Will Schott: "Re: LSR II - scales/balances to track <BR/>usage of sheath" <BR/>Next in thread: Nilles, Tricia L..: "RE: Diva 6.1.1 user issues" <BR/>Reply: Nilles, Tricia L..: "RE: Diva 6.1.1 user issues" <BR/>Reply: Linkes, Sean Patrick: "RE: Diva 6.1.1 user issues" <BR/>Reply: Roberts, Daniel: "RE: Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Fri Oct 17 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>RE: Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] [ Next in thread ] [ Replies ] <BR/>From: Nilles, Tricia L.. tnil...@jhsph.edu <BR/>Date: Wed Oct 15 2008 - 09:43:57 EDT <BR/><BR/><BR/>Wayne, <BR/><BR/><BR/>Yes, we also have experienced a similar issue. After running CS&T, <BR/>we <BR/>cannot make any <BR/>changes (ie renaming specimens, experiments) we first have to exit <BR/>and <BR/>relaunch the <BR/>software. Logging out does not work. I have discussed this with our <BR/>local service rep <BR/>and also one of the software specialists who came in for local <BR/>training and demos, and no <BR/>one has offered a 'fix'. <BR/><BR/><BR/>Tricia L Nilles <BR/>Research Specialist <BR/>Flow Cytometry Laboratory <BR/>Johns Hopkins Bloomberg School of Public Health <BR/>Phone: 410-502-9290 <BR/>Fax: 410-614-2503 <BR/>- <BR/><BR/><BR/><BR/> <BR/><BR/>-----Original Message----- <BR/>From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk] <BR/>Sent: Tuesday, October 14, 2008 9:32 AM <BR/>To: cyto-inbox <BR/>Subject: Diva 6.1.1 user issues <BR/><BR/>Afternoon flowers, <BR/><BR/><BR/>Having recently moved to Diva 6.1.1, we're experiencing some software <BR/>lock-up problems, usually after closing down CS&T, that require a <BR/>full re-install, which is becoming tiresome, and I wondered if any <BR/>others had experienced similar problems? <BR/><BR/><BR/>Wayne <BR/><BR/><BR/>Wayne Turnbull <BR/>Flow Cytometry Services <BR/>Department of Immunobiology <BR/>GKT Guy's Hospital <BR/>London SE1 9RT <BR/>Received on Wed Oct 15 12:38:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Julie Nelson: "MoFlo pressure issues" <BR/>Previous message: Brian Binder: "RE: Make & Model of this Flow <BR/>Cytometer anyone? Circa 1991" <BR/>In reply to: Wayne Turnbull: "Diva 6.1.1 user issues" <BR/>Next in thread: Berend: "Re: Diva 6.1.1 user issues" <BR/>Reply: Berend: "Re: Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Fri Oct 17 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>Re: Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] [ Next in thread ] [ Replies ] <BR/>From: Berend b.hooibr...@amc.uva.nl <BR/>Date: Thu Oct 16 2008 - 03:58:54 EDT <BR/><BR/><BR/>We have seen similar problems with 6.1.1 and CST module. I am not <BR/>able to run the cst when I started the diva software in a Windows <BR/>(normal)User account. It locks-up immediately. So, we first log in <BR/>as <BR/>administrator in windows xp, do the cst run, quit diva after this qc- <BR/>check and log in as (limited) user. Thereafter we startup diva again <BR/>and everything works fine, except the cst and configuration modules. <BR/>Our service engineer (BD) couldn't fix it so far. Still I prefer the <BR/>let everybody work as limited user with the LSR, to keep systems <BR/>clean from al kind of ?%^&b 9b b " people want to install. <BR/>Berend <BR/><BR/><BR/>On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote: <BR/><BR/><BR/>> Wayne, <BR/><BR/><BR/>> Yes, we also have experienced a similar issue. After running CS&T, <BR/>> we cannot make any <BR/>> changes (ie renaming specimens, experiments) we first have to exit <BR/>> and relaunch the <BR/>> software. Logging out does not work. I have discussed this with <BR/>> our local service rep <BR/>> and also one of the software specialists who came in for local <BR/>> training and demos, and no <BR/>> one has offered a 'fix'. <BR/><BR/><BR/>> Tricia L Nilles <BR/>> Research Specialist <BR/>> Flow Cytometry Laboratory <BR/>> Johns Hopkins Bloomberg School of Public Health <BR/>> Phone: 410-502-9290 <BR/>> Fax: 410-614-2503 <BR/><BR/><BR/>> -----Original Message----- <BR/>> From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk] <BR/>> Sent: Tuesday, October 14, 2008 9:32 AM <BR/>> To: cyto-inbox <BR/>> Subject: Diva 6.1.1 user issues <BR/><BR/><BR/>> Afternoon flowers, <BR/><BR/><BR/>> Having recently moved to Diva 6.1.1, we're experiencing some software <BR/>> lock-up problems, usually after closing down CS&T, that require a <BR/>> full re-install, which is becoming tiresome, and I wondered if any <BR/>> others had experienced similar problems? <BR/><BR/><BR/>> Wayne <BR/><BR/><BR/>> Wayne Turnbull <BR/>> Flow Cytometry Services <BR/>> Department of Immunobiology <BR/>> GKT Guy's Hospital <BR/>> London SE1 9RT <BR/><BR/><BR/>Berend Hooibrink <BR/>Flow cytometry core facility <BR/>Dept.. Cellbiology & Histology room L3-115 <BR/>AMC <BR/>Meibergdreef 15 <BR/>1105AZ Amsterdam <BR/>Netherlands <BR/>Tel.: 31(0)20-5666804 <BR/>Fax: 31(0)20-6974156 <BR/>Received on Thu Oct 16 15:38:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Dave Humphries: "RE: [ Fluorescence amplifier? ]" <BR/>Previous message: Leipziger Workshop: "13th Leipziger Workshop, <BR/>Meeting Report published; 14th Leipziger Workshop announcenment" <BR/>In reply to: Nilles, Tricia L..: "RE: Diva 6.1.1 user issues" <BR/>Next in thread: Ted Hibbs: "RE: Diva 6.1.1 user issues" <BR/>Reply: Ted Hibbs: "RE: Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Sat Oct 18 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>RE: Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] [ Next in thread ] <BR/>From: Ted Hibbs Ted_Hi...@bonfils.org <BR/>Date: Thu Oct 16 2008 - 22:27:10 EDT <BR/><BR/><BR/>Berend, <BR/><BR/><BR/>We had a similar issue when we first got our BD CantoII with Diva <BR/>6.1.1. It <BR/>is related to the permissions on the folders on the C:\ drive, though <BR/>I donb t <BR/>remember exactly where. Once I changed the permissions on the <BR/>folders, it is <BR/>working well. <BR/><BR/><BR/>Ted <BR/><BR/><BR/>From: Berend [mailto:b.hooibr...@amc.uva.nl] <BR/>Sent: Thursday, October 16, 2008 1:59 AM <BR/>To: Cytometry Mailing List <BR/>Subject: Re: Diva 6.1.1 user issues <BR/><BR/><BR/>We have seen similar problems with 6.1.1 and CST module. I am not <BR/>able <BR/>to run <BR/>the cst when I started the diva software in a Windows (normal)User <BR/>account. <BR/>It locks-up immediately. So, we first log in as administrator in <BR/>windows xp, <BR/>do the cst run, quit diva after this qc-check and log in as (limited) <BR/>user. <BR/>Thereafter we startup diva again and everything works fine, except <BR/>the <BR/>cst <BR/>and configuration modules. Our service engineer (BD) couldn't fix it <BR/>so far. <BR/>Still I prefer the let everybody work as limited user with the LSR, <BR/>to <BR/>keep <BR/>systems clean from al kind of ?%^&b 9b b " people want to install. <BR/><BR/><BR/>Berend <BR/><BR/><BR/>On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote: <BR/><BR/><BR/>Wayne, <BR/><BR/><BR/>Yes, we also have experienced a similar issue. After running CS&T, we <BR/>cannot <BR/>make any <BR/><BR/><BR/>changes (ie renaming specimens, experiments) we first have to exit <BR/>and <BR/>relaunch the <BR/><BR/><BR/>software. Logging out does not work. I have discussed this with our <BR/>local <BR/>service rep <BR/><BR/><BR/>and also one of the software specialists who came in for local <BR/>training and <BR/>demos, and no <BR/><BR/><BR/>one has offered a 'fix'. <BR/><BR/><BR/>Tricia L Nilles <BR/><BR/><BR/>Research Specialist <BR/><BR/><BR/>Flow Cytometry Laboratory <BR/><BR/><BR/>Johns Hopkins Bloomberg School of Public Health <BR/><BR/><BR/>Phone: 410-502-9290 <BR/><BR/><BR/>Fax: 410-614-2503 <BR/><BR/><BR/>-----Original Message----- <BR/><BR/><BR/>From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk] <BR/><BR/><BR/>Sent: Tuesday, October 14, 2008 9:32 AM <BR/><BR/><BR/>To: cyto-inbox <BR/><BR/><BR/>Subject: Diva 6.1.1 user issues <BR/><BR/><BR/>Afternoon flowers, <BR/><BR/><BR/>Having recently moved to Diva 6.1.1, we're experiencing some software <BR/><BR/><BR/>lock-up problems, usually after closing down CS&T, that require a <BR/><BR/><BR/>full re-install, which is becoming tiresome, and I wondered if any <BR/><BR/><BR/>others had experienced similar problems? <BR/><BR/><BR/>Wayne <BR/><BR/><BR/>Wayne Turnbull <BR/><BR/><BR/>Flow Cytometry Services <BR/><BR/><BR/>Department of Immunobiology <BR/><BR/><BR/>GKT Guy's Hospital <BR/><BR/><BR/>London SE1 9RT <BR/><BR/><BR/>Berend Hooibrink <BR/><BR/><BR/>Flow cytometry core facility <BR/><BR/><BR/>Dept.. Cellbiology & Histology room L3-115 <BR/><BR/><BR/>AMC <BR/><BR/><BR/>Meibergdreef 15 <BR/><BR/><BR/>1105AZ Amsterdam <BR/><BR/><BR/>Netherlands <BR/><BR/><BR/>Tel.: 31(0)20-5666804 <BR/><BR/><BR/>Fax: 31(0)20-6974156 <BR/><BR/><BR/>Confidentiality Notice: This e-mail message, including any <BR/>attachments, is for the sole use of the intended recipient(s) and may <BR/>contain confidential and privileged information and must be protected <BR/>in accordance with those provisions. Any unauthorized review, use, <BR/>disclosure or distribution is prohibited. If you are not the <BR/>intended <BR/>recipient, please contact the sender by reply e-mail and destroy all <BR/>copies of the original message. <BR/>Received on Fri Oct 17 13:18:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Nawroly, Niga S: "Job-Imperial college London- Flow <BR/>Cytometry" <BR/>Previous message: Gerstein, Rachel: "RE: FACS cell sorting- Azide <BR/>free <BR/>antibodies" <BR/>In reply to: Berend: "Re: Diva 6.1.1 user issues" <BR/>Next in thread: Linkes, Sean Patrick: "RE: Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Sat Oct 18 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>RE: Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] <BR/>From: Linkes, Sean Patrick sean.lin...@utoledo.edu <BR/>Date: Tue Oct 14 2008 - 16:11:56 EDT <BR/><BR/><BR/>No problems here. Our CS&T works wonderfully. Sometimes the setup <BR/>doesn't work right even with a freshly prepared tube of beads (with <BR/>the BD Field Service guy leaving one day prior having aligned the <BR/>lasers). Sean <BR/><BR/><BR/>Sean Linkes MS <BR/>Research Associate <BR/>Flow Cytometry Core Manager <BR/>The University of Toledo HSC <BR/>(419) 383 3402 http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html <BR/><BR/><BR/>________________________________ <BR/><BR/><BR/>From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk] <BR/>Sent: Tue 10/14/2008 9:32 AM <BR/>To: cyto-inbox <BR/>Subject: Diva 6.1.1 user issues <BR/><BR/><BR/>Afternoon flowers, <BR/><BR/><BR/>Having recently moved to Diva 6.1.1, we're experiencing some software <BR/>lock-up problems, usually after closing down CS&T, that require a <BR/>full re-install, which is becoming tiresome, and I wondered if any <BR/>others had experienced similar problems? <BR/><BR/><BR/>Wayne <BR/><BR/><BR/>Wayne Turnbull <BR/>Flow Cytometry Services <BR/>Department of Immunobiology <BR/>GKT Guy's Hospital <BR/>London SE1 9RT <BR/>Received on Wed Oct 15 14:18:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Cappella, Paolo Elia [Nervianoms]: "FCS generator" <BR/>Previous message: Leo_Bu...@bd.com: "Re: Make & Model of this Flow <BR/>Cytometer anyone? Circa 1991" <BR/>In reply to: Wayne Turnbull: "Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Thu Oct 16 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>RE: Diva 6.1.1 user issues <BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] <BR/>From: Roberts, Daniel Daniel.Robe...@covance.com <BR/>Date: Wed Oct 15 2008 - 18:53:42 EDT <BR/><BR/><BR/> I've been using 6.1.1 since May - no problems here. I'd question <BR/>your <BR/>Ram, <BR/>but if you bought the computer as part of the package - you should be <BR/>OK. I <BR/>know that sometimes leaving the beads on there can cause issues - but <BR/>If <BR/>memory serves (as opposed to habit) the CST software prompts/makes <BR/>you <BR/>take <BR/>the beads off. Other than that, I can't think of why it wouldn't <BR/>reconnect <BR/>to Diva. <BR/><BR/><BR/>Dan <BR/>Technical Resource Specialist <BR/>Covance Labs, NA <BR/>Vienna, VA 20850 <BR/><BR/><BR/>-----Original Message----- <BR/>From: Linkes, Sean Patrick <BR/>To: cyto-inbox <BR/>Sent: 10/14/2008 3:11 PM <BR/>Subject: RE: Diva 6.1.1 user issues <BR/><BR/><BR/>No problems here. Our CS&T works wonderfully. Sometimes the setup <BR/>doesn't work right even with a freshly prepared tube of beads (with <BR/>the <BR/>BD Field Service guy leaving one day prior having aligned the <BR/>lasers). <BR/>Sean <BR/>Sean Linkes MS <BR/>Research Associate <BR/>Flow Cytometry Core Manager <BR/>The University of Toledo HSC <BR/>(419) 383 3402 <BR/> http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html <BR/><BR/><BR/> _____ <BR/><BR/><BR/>From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk] <BR/>Sent: Tue 10/14/2008 9:32 AM <BR/>To: cyto-inbox <BR/>Subject: Diva 6.1.1 user issues <BR/><BR/><BR/>Afternoon flowers, <BR/><BR/><BR/>Having recently moved to Diva 6.1.1, we're experiencing some software <BR/>lock-up problems, usually after closing down CS&T, that require a <BR/>full re-install, which is becoming tiresome, and I wondered if any <BR/>others had experienced similar problems? <BR/><BR/><BR/>Wayne <BR/><BR/><BR/>Wayne Turnbull <BR/>Flow Cytometry Services <BR/>Department of Immunobiology <BR/>GKT Guy's Hospital <BR/>London SE1 9RT <BR/><BR/><BR/>----------------------------------------------------- <BR/>Confidentiality Notice: This e-mail transmission <BR/>may contain confidential or legally privileged <BR/>information that is intended only for the individual <BR/>or entity named in the e-mail address. If you are not <BR/>the intended recipient, you are hereby notified that <BR/>any disclosure, copying, distribution, or reliance <BR/>upon the contents of this e-mail is strictly prohibited. <BR/><BR/><BR/>If you have received this e-mail transmission in error, <BR/>please reply to the sender, so that we can arrange <BR/>for proper delivery, and then please delete the message <BR/>from your inbox. Thank you. <BR/>Received on Thu Oct 16 14:18:00 2008 <BR/>This message: [ Message body ] <BR/>Next message: Stetler-Stevenson, Maryalice (NIH/NCI) [E]: "Paula <BR/>Imus" <BR/>Previous message: David Galbraith: "Fwd: Re: 2008 Nobel Prize in <BR/>Chemistry" <BR/>In reply to: Wayne Turnbull: "Diva 6.1.1 user issues" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By <BR/>Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Fri Oct 17 2008 - <BR/>03:12:06 EDT <BR/><BR/><BR/>If anyone is interested in their software to function properly please <BR/>contact Kanecki Associates Inc. <BR/><BR/><BR/>Http://www.kanecki.com <BR/>Ceo@kanecki.com <BR/>Mitch@kanecki.com <BR/><BR/><BR/>Mitchell Haynes <BR/>Vp. <BR/>Kanecki Associates Inc <BR/>281-444-1619 <BR/><BR/><BR/>Thank youAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-11685527021113770452008-10-15T00:12:00.000-05:002008-10-15T00:12:00.000-05:00Purdue University Cytometry Labatories & Mail ...Purdue University Cytometry Labatories & Mail List <BR/>RE: Bad Flow Data & reviewing -- What can we do? From: Claudio Vallan <BR/>(claudio.val...@dkf7.unibe.ch) Date: Thu Oct 25 2001 - 02:39:08 EST • <BR/>Next message: Howard <BR/>Shapiro: "Re:Virus Sorting" • Previous message: Maciej Simm: "Re: Bad <BR/>Flow data thread" • In reply to: Gerhard Nebe-von-Caron: "RE: Bad <BR/>Flow <BR/>Data & reviewing -- What can we do?" • Next in thread: sterling <BR/>stoudenmire: "RE: Bad Flow Data & reviewing -- What can we do?" • <BR/>Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] ________________________________________ <BR/><BR/>Maybe <BR/><BR/><BR/>it is not a bad idea to make post print reviews, white papers, <BR/>instructions to authors etc. etc. <BR/>But I think al of this will be mainly for <BR/><BR/><BR/>our own use. "Normal" scientist will probably never know that such <BR/>stuff even exists. <BR/><BR/><BR/>I think that the only way to make scientist aware of the problems <BR/>thay <BR/>may encounter when generating cytometric <BR/>datas <BR/><BR/><BR/>is to infiltrate their meetings and conferences and have the guts to <BR/>stand up <BR/><BR/><BR/>and tell them that what they are showing on that slide is just <BR/>bullshit. <BR/><BR/><BR/>If that happens a few time they will certainly have a closer look at <BR/>what they are doing. <BR/><BR/><BR/>Though, I do not think they will take us very seriously if we just <BR/>tell them that they have mislabelled the axes. <BR/><BR/><BR/>Claudio Vallan =================================================== <BR/>Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone <BR/>Office: 031 / 632 99 68 University of Bern E-Mail: <BR/>val...@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31 <BR/>Insel <BR/>hosptial area only: 3010 <BR/>Bern Beeper: 181 67 59 <BR/>\ <BR/>On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-65439451962598334892008-10-14T23:36:00.000-05:002008-10-14T23:36:00.000-05:00RE: ISAC - MultiColor Flow CytometryThis message: ...RE: ISAC - MultiColor Flow Cytometry<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] <BR/><BR/>From: Marty Bigos mbigos@gladstone.ucsf.edu<BR/><BR/>Date: Tue Apr 27 2004 - 19:21:42 EST<BR/><BR/>Just to give credit where it is due:<BR/><BR/>The presentations at this workshop were (in order of appearance):<BR/><BR/>Marty Bigos - Gladstone Institutes - Basic Concepts for Compensation<BR/><BR/>Mario Roederer - NIH - The Hard, the Bad, and the Ugly<BR/><BR/>Nicole Baumgarth - UC Davis - The "How-to Guide" to Compensation for <BR/><BR/>Multicolor Cytometry<BR/><BR/>Panel - the above 3 joined by Bill Hyun (UCSF) and David Parks (Stanford)<BR/><BR/>The workshop was organized by Treestar, Inc. and the Gladstone Flow <BR/><BR/>Core. Many companies contributed to making it happen (in alphabetical <BR/><BR/>order):<BR/><BR/>Amnis, BD Biosciences, Beckman-Coulter, Caltag, DakoCytomation, <BR/><BR/>DeNovo Software, FloCyte Associates, Miltenyi, and Southern BioTech.<BR/><BR/>Paul Robinson and Bartek Rajwa did the video post-production.<BR/><BR/>Marty<BR/><BR/>>The question has been asked about videoing ISAC<BR/><BR/>>workshops and tutorials. On CD8 which will be coming out<BR/><BR/>>at ISAC, we have a bonus. CD8 is a 2 cd set and the<BR/><BR/>>second CD is an outstanding tutorial that Mario and Marty<BR/><BR/>>Bigos and others put on in San Francisco last year. Its really<BR/><BR/>>good!<BR/><BR/>><BR/><BR/>>We would like to do this for selected tutorials even at ISAC<BR/><BR/>>and we are developing a proposal for ISAC as we speak. It<BR/><BR/>>would help to know if this was something of interest.<BR/><BR/>><BR/><BR/>>Regards<BR/><BR/>>paul robinson<BR/><BR/>>Purdue<BR/><BR/>><BR/><BR/>><BR/><BR/>><BR/><BR/>>On 26 Apr 2004 at 11:03, Kroeger, Jodi wrote:<BR/><BR/>><BR/><BR/>>> What are the chances that this tutorial, (and others of high <BR/><BR/>>>interest) could be<BR/><BR/>>> video taped and broadcasted over the internet for those <BR/><BR/>>>unfortunate souls unable<BR/><BR/>>> to attend? At least to those of us who are ISAC members?<BR/><BR/>>><BR/><BR/>>> -----Original Message-----<BR/><BR/>>> From: Mario Roederer [mailto:roederer@drmr.com]<BR/><BR/>>> Sent: Thursday, April 22, 2004 11:48 PM<BR/><BR/>>> To: cyto-inbox<BR/><BR/>>> Subject: ISAC - MultiColor Flow Cytometry<BR/><BR/>>><BR/><BR/>>><BR/><BR/>>> For the second straight ISAC (officially making this a tradition),<BR/><BR/>>> I'll be running a Saturday tutorial on Multicolor Flow Cytometry.<BR/><BR/>>> This time, instead of being at the unhappy hour of 8 in the morning,<BR/><BR/>>> it will be at a much more civilized early afternoon time.<BR/><BR/>>><BR/><BR/>>> This tutorial (aptly numbered: Tutorial "X") will be much more<BR/><BR/>>> pragmatic in nature than previous. We will have three talks that<BR/><BR/>>> will center on how to plan and implement a multicolor<BR/><BR/>>> immunophenotyping experiment, including instrument setup and<BR/><BR/>>> validation (Steve Perfetto), multicolor panel design and optimization<BR/><BR/>>> (myself), and bridging the gap to the clinic (Brent Wood). Assuming<BR/><BR/>>> that most of us are not in a full post-prandial siesta mode, this<BR/><BR/>>> should be a highly interactive session: please come with your<BR/><BR/>>> questions, data, artefacts, and complaints in hand!<BR/><BR/>>><BR/><BR/>>> If you are jumping on the 7, 10, or 15-color bandwagon, then this<BR/><BR/>>> tutorial is for you! If you've never done more than 2-color<BR/><BR/>>> staining, then enjoy the wonderful city of Montpellier instead :)<BR/><BR/>>><BR/><BR/>>> If you have any suggestions for specific topics, or any general<BR/><BR/>>> questions, please send them to me in advance and we will try to<BR/><BR/>>> address them.<BR/><BR/>>><BR/><BR/>>> Regards,<BR/><BR/>>><BR/><BR/>>> mr<BR/><BR/>>> ######################################################################<BR/><BR/>>> This transmission may be confidential or protected from disclosure and<BR/><BR/>>> is only for review and use by the intended recipient. Access by<BR/><BR/>>> anyone else is unauthorized. Any unauthorized reader is hereby<BR/><BR/>>> notified that any review, use, dissemination, disclosure or copying of<BR/><BR/>>> this information, or any act or omission taken in reliance on it, is<BR/><BR/>>> prohibited and may be unlawful. If you received this transmission in<BR/><BR/>>> error, please notify the sender immediately. Thank you.<BR/><BR/>>><BR/><BR/>>> ######################################################################<BR/><BR/>>><BR/><BR/>><BR/><BR/>><BR/><BR/>>J.Paul Robinson, PhD PH:(765)4940757<BR/><BR/>>Professor of Immunopharmacology<BR/><BR/>>Professor of Biomedical Engineering <BR/><BR/>>Purdue University FAX:(765)4940517<BR/><BR/>>EMAIL:jpr@flowcyt.cyto.purdue.edu<BR/><BR/>>WEB: http://www.cyto.purdue.edu <BR/><BR/>><BR/><BR/>>Have you seen our new HCS webpage?<BR/><BR/>>http://www.cyto.purdue.edu/hcs<BR/><BR/>Received on Wed Apr 28 16:56:53 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: Christopher J. Groves: "Re: Creating a database of FCS files" <BR/><BR/>Previous message: Cris Bare: "Re: RFI's on longitudinal data (question restated)" <BR/><BR/>In reply to: J. Paul Robinson: "RE: ISAC - MultiColor Flow Cytometry" <BR/><BR/>Next in thread: Jennifer Wilshire: "More Credit for Comp Workshop Video" <BR/><BR/>Reply: Jennifer Wilshire: "More Credit for Comp Workshop Video" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Sat May 01 2004 - 03:12:03 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-69132272790009209632008-10-14T23:32:00.000-05:002008-10-14T23:32:00.000-05:00From: Marty Bigos mbigos@gladstone.ucsf.edu Date: ...From: Marty Bigos mbigos@gladstone.ucsf.edu <BR/><BR/>Date: Tue Apr 27 2004 - 11:05:04 EST<BR/><BR/>Hi Adrian -<BR/><BR/>The need of a management system for flow cytometry (as well as <BR/><BR/>microscope image) data has been apparent for quite some time. Not <BR/><BR/>only would it help in the research aspects you mentioned, it could <BR/><BR/>also provide many other functions, such as secured sharing of data, <BR/><BR/>easy availability from any networked site, etc.<BR/><BR/>For several years I have been fortunate to be involved with Bill Hyun <BR/><BR/>of UCSF through an SBIR with a local software company in SF (Biotrue, <BR/><BR/>Inc.) to develop such a system. We are in beta test now.<BR/><BR/>The product, (which doesn't have a formal name yet, but is informally <BR/><BR/>called RDMS - Research Data Management System), will be announced at <BR/><BR/>the ISAC meeting at the end of May. Biotrue will have a booth there <BR/><BR/>to give demos of the product, and I will be giving a workshop talk on <BR/><BR/>some of the ideas behind its design.<BR/><BR/>I do have a (small) financial interest in Biotrue, but even if I <BR/><BR/>didn't I would recommend that you look at the product as a possible <BR/><BR/>solution to your needs. You can contact Jenny Liu (JLiu@Biotrue.Net) <BR/><BR/>for more information.<BR/><BR/>Marty<BR/><BR/>Marty Bigos<BR/><BR/>Director, Flow Core<BR/><BR/>Gladstone Institute of Virology and Immunology<BR/><BR/>Building 3 SFGH Rm 509<BR/><BR/>415-695-3832<BR/><BR/>>Hi all,<BR/><BR/>><BR/><BR/>>Some of the users here have raised the desirability of having a <BR/><BR/>>database of all the FCS headers from all their data files. They <BR/><BR/>>could then, for example, search for all the files/experiments in <BR/><BR/>>which they used a particular stain etc.<BR/><BR/>><BR/><BR/>>Is anybody doing this? Would this be something that other people <BR/><BR/>>would find useful?<BR/><BR/>><BR/><BR/>>I would love to set something up but I don't have the requisite <BR/><BR/>>skills or time at the moment.<BR/><BR/>><BR/><BR/>>As a temporary measure I suggested they export the FCS header info <BR/><BR/>>from FlowJo using using a table and then compile them all in another <BR/><BR/>>program like excel. This works for a few experiments but it needs to <BR/><BR/>>be automated (and easy) if it is going to be generally applicable.<BR/><BR/>><BR/><BR/>>Any comments or suggestions?<BR/><BR/>><BR/><BR/>>Adrian Smith<BR/><BR/>>Centenary Institute of Cancer Medicine and Cell Biology<BR/><BR/>>Sydney, Australia<BR/><BR/>-- <BR/><BR/>Received on Tue Apr 27 13:58:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: J. Paul Robinson: "RE: ISAC - MultiColor Flow Cytometry" <BR/><BR/>Previous message: Szmania, Susann M: "[Reagent source?]" <BR/><BR/>In reply to: Adrian Smith: "Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Wed Apr 28 2004 - 03:12:04 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-87068411895516785972008-10-14T22:55:00.000-05:002008-10-14T22:55:00.000-05:00RE: Creating a database of FCS filesThis message: ...RE: Creating a database of FCS files<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] [ In reply to ] <BR/><BR/>From: Robert C. Leif rleif@rleif.com <BR/><BR/>Date: Sat Apr 24 2004 - 11:57:17 EST<BR/><BR/>The FCS header files have already been parsed and stored in a database(1).<BR/><BR/>The product was QCTracker from Phoenix Flow Systems.<BR/><BR/>Experience with the development of that product was one of the reasons for<BR/><BR/>the creation of CytometryML. Since the data in a CytometryML file is all<BR/><BR/>validated XML, it can be imported without any changes into commercially<BR/><BR/>available databases, spreadsheets and other applications. The list mode<BR/><BR/>data and associated index files are stored as a simple array of<BR/><BR/>records(structs), which can be read by commercially available common<BR/><BR/>programming languages or manipulated by .Net object. <BR/><BR/>1. R. C. Leif, R. Rios, M. C. Becker, C. K. Becker, J. T. Self, and S. B.<BR/><BR/>Leif, "The Creation of a Laboratory Instrument Quality Monitoring System<BR/><BR/>with AdaSAGE". Advanced Techniques in Analytical Cytology, Optical Diagnosis<BR/><BR/>of Living Cells and Biofluids, Ed. T. Askura, D. L. Farkas, R. C. Leif, A.<BR/><BR/>V. Priezzhev, , and B. J. Tromberg.. A. Katzir Progress in Biomedical Optics<BR/><BR/>Series Editor SPIE Proceedings Series, Vol. 2678, 232-239 (1996).<BR/><BR/>2. R. C. Leif, S. B. Leif, and S. H. Leif, "CytometryML, An XML Format based<BR/><BR/>on DICOM for Analytical Cytology Data ", Cytometry 54A pp. 56-65 (2003).<BR/><BR/>3. R.C. Leif, S.H. Leif, S.B. Leif, CytometryML, a markup language for<BR/><BR/>analytical cytology, in Manipulation and Analysis of Biomolecules, Cells and<BR/><BR/>Tissues, D. V. Nicolau, J. Enderlein, and R. C. Leif, Editors, SPIE<BR/><BR/>Proceedings Vol. 4962 pp 288-297 (2003).<BR/><BR/>-----Original Message-----<BR/><BR/>From: Adrian Smith [mailto:A.Smith@centenary.usyd.edu.au] <BR/><BR/>Sent: Thursday, April 22, 2004 6:57 PM<BR/><BR/>To: cyto-inbox<BR/><BR/>Subject: Creating a database of FCS files<BR/><BR/>Hi all,<BR/><BR/>Some of the users here have raised the desirability of having a <BR/><BR/>database of all the FCS headers from all their data files. They could <BR/><BR/>then, for example, search for all the files/experiments in which they <BR/><BR/>used a particular stain etc.<BR/><BR/>Is anybody doing this? Would this be something that other people <BR/><BR/>would find useful?<BR/><BR/>I would love to set something up but I don't have the requisite <BR/><BR/>skills or time at the moment.<BR/><BR/>As a temporary measure I suggested they export the FCS header info <BR/><BR/>from FlowJo using using a table and then compile them all in another <BR/><BR/>program like excel. This works for a few experiments but it needs to <BR/><BR/>be automated (and easy) if it is going to be generally applicable.<BR/><BR/>Any comments or suggestions?<BR/><BR/>Adrian Smith<BR/><BR/>Centenary Institute of Cancer Medicine and Cell Biology<BR/><BR/>Sydney, Australia<BR/><BR/>Received on Mon Apr 26 14:38:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: Stojan Dimitrov: "anti-IL-6 antibody against not-recombinant IL-6" <BR/><BR/>Previous message: Kroeger, Jodi: "RE: ISAC - MultiColor Flow Cytometry" <BR/><BR/>In reply to: Adrian Smith: "Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Tue Apr 27 2004 - 03:12:02 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-53150980318360224902008-10-14T22:52:00.000-05:002008-10-14T22:52:00.000-05:00Re: Creating a database of FCS filesThis message: ...Re: Creating a database of FCS files<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] <BR/><BR/>From: David Novo dave@denovosoftware.com <BR/><BR/>Date: Fri Apr 23 2004 - 19:52:09 EST<BR/><BR/>Hello Adrian,<BR/><BR/>If you are willing to use a PC, then FCS Express (www.denovosoftware.com) <BR/><BR/>can do what you need. You can choose which keywords you are interested in <BR/><BR/>and then automatically export them to Excel. Each keyword will become a <BR/><BR/>different column and each data file will have its own row. You can choose <BR/><BR/>which files you are interested in processing, and FCS Express will <BR/><BR/>automatically extract the keywords you are interested in and place them in <BR/><BR/>the spreadsheet.<BR/><BR/>-Dave<BR/><BR/>At 06:56 PM 4/22/2004, Adrian Smith wrote:<BR/><BR/>>Hi all,<BR/><BR/>><BR/><BR/>>Some of the users here have raised the desirability of having a database <BR/><BR/>>of all the FCS headers from all their data files. They could then, for <BR/><BR/>>example, search for all the files/experiments in which they used a <BR/><BR/>>particular stain etc.<BR/><BR/>><BR/><BR/>>Is anybody doing this? Would this be something that other people would <BR/><BR/>>find useful?<BR/><BR/>><BR/><BR/>>I would love to set something up but I don't have the requisite skills or <BR/><BR/>>time at the moment.<BR/><BR/>><BR/><BR/>>As a temporary measure I suggested they export the FCS header info from <BR/><BR/>>FlowJo using using a table and then compile them all in another program <BR/><BR/>>like excel. This works for a few experiments but it needs to be automated <BR/><BR/>>(and easy) if it is going to be generally applicable.<BR/><BR/>><BR/><BR/>>Any comments or suggestions?<BR/><BR/>><BR/><BR/>>Adrian Smith<BR/><BR/>>Centenary Institute of Cancer Medicine and Cell Biology<BR/><BR/>>Sydney, Australia<BR/><BR/>------------------------------<BR/><BR/>David Novo<BR/><BR/>President<BR/><BR/>De Novo Software<BR/><BR/>www.denovosoftware.com<BR/><BR/>phone: (310) 558-4955<BR/><BR/>fax: (310) 943-1489<BR/><BR/>Received on Mon Apr 26 13:18:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: jschmitz@bidmc.harvard.edu: "RE: Antibody titrations (2004)" <BR/><BR/>Previous message: facs_copy@wehi.EDU.AU: "Re: histogram raw data" <BR/><BR/>In reply to: Adrian Smith: "Creating a database of FCS files" <BR/><BR/>Next in thread: Robert C. Leif: "RE: Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Tue Apr 27 2004 - 03:12:02 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-10036829373821375702008-10-14T22:51:00.000-05:002008-10-14T22:51:00.000-05:00From: Adrian Smith A.Smith@centenary.usyd.edu.AU D...From: Adrian Smith A.Smith@centenary.usyd.edu.AU <BR/><BR/>Date: Thu Apr 22 2004 - 20:56:40 EST<BR/><BR/>Hi all,<BR/><BR/>Some of the users here have raised the desirability of having a <BR/><BR/>database of all the FCS headers from all their data files. They could <BR/><BR/>then, for example, search for all the files/experiments in which they <BR/><BR/>used a particular stain etc.<BR/><BR/>Is anybody doing this? Would this be something that other people <BR/><BR/>would find useful?<BR/><BR/>I would love to set something up but I don't have the requisite <BR/><BR/>skills or time at the moment.<BR/><BR/>As a temporary measure I suggested they export the FCS header info <BR/><BR/>from FlowJo using using a table and then compile them all in another <BR/><BR/>program like excel. This works for a few experiments but it needs to <BR/><BR/>be automated (and easy) if it is going to be generally applicable.<BR/><BR/>Any comments or suggestions?<BR/><BR/>Adrian Smith<BR/><BR/>Centenary Institute of Cancer Medicine and Cell Biology<BR/><BR/>Sydney, Australia<BR/><BR/>Received on Fri Apr 23 15:38:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: Mario Roederer: "ISAC - MultiColor Flow Cytometry" <BR/><BR/>Previous message: Dorothea Torous: "histogram raw data" <BR/><BR/>Next in thread: David Novo: "Re: Creating a database of FCS files" <BR/><BR/>Reply: David Novo: "Re: Creating a database of FCS files" <BR/><BR/>Reply: Robert C. Leif: "RE: Creating a database of FCS files" <BR/><BR/>Reply: Marty Bigos: "Re: Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Wed Apr 28 2004 - 03:12:04 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-51372992553634933722008-10-14T22:50:00.000-05:002008-10-14T22:50:00.000-05:00Creating a database of FCS filesThis message: [ Me...Creating a database of FCS files<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] [ Next in thread ] [ Replies ] <BR/><BR/>From: VSH - Tech Support tech@vsh.com<BR/><BR/>Date: Tue Apr 27 2004 - 09:00:36 EST<BR/><BR/>Hello, Flow-ers,<BR/><BR/>With reference to databasing FCS file keywords, WinList from Verity Software<BR/><BR/>has full databasing capability. The user may choose FCS file keywords and<BR/><BR/>results keywords to database in a delimited ASCII text format. The database<BR/><BR/>can be easily imported into Excel and similar programs. WinList supports<BR/><BR/>batch processing, and is available for PC and Mac platforms. For more<BR/><BR/>information and to download a trial version, visit our web site at<BR/><BR/>www.vsh.com.<BR/><BR/>Mark Munson<BR/><BR/>Sales Manager, Verity Software House.<BR/><BR/>Received on Tue Apr 27 12:58:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: VSH - Tech Support: "histogram raw data" <BR/><BR/>Previous message: James Mittler: "Hoechst on Aria" <BR/><BR/>Next in thread: Smoot, Doug: "RE: Creating a database of FCS files" <BR/><BR/>Maybe reply: Smoot, Doug: "RE: Creating a database of FCS files" <BR/><BR/>Maybe reply: Ryan Brinkman: "Re: Creating a database of FCS files" <BR/><BR/>Maybe reply: Christopher J. Groves: "Re: Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Thu Apr 29 2004 - 03:12:03 EST <BR/><BR/>October 5, 2008 6:28 AMAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-47011470536535147682008-10-14T22:49:00.000-05:002008-10-14T22:49:00.000-05:00histogram raw dataThis message: [ Message body ] [...histogram raw data<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] <BR/><BR/>From: VSH - Tech Support tech@vsh.com <BR/><BR/>Date: Tue Apr 27 2004 - 09:02:24 EST<BR/><BR/>Hello, Flow-ers,<BR/><BR/>WinList software, for PC or for Mac, can export FCS file data in a<BR/><BR/>tab-delimited ASCII text format. You may choose any or all parameters for<BR/><BR/>export, raw and/or compensated data, and gating may be applied as well. For<BR/><BR/>more information, please see our web site at www.vsh.com or contact me<BR/><BR/>directly.<BR/><BR/>Best regards,<BR/><BR/>Mark<BR/><BR/>Mark E. Munson<BR/><BR/>Sales Manager<BR/><BR/>Verity Software House, Inc.<BR/><BR/>45A Augusta Road<BR/><BR/>PO Box 247<BR/><BR/>Topsham, ME 04086<BR/><BR/>Phone: 207-729-6767 x191<BR/><BR/>Fax: 207-729-5443<BR/><BR/>Received on Tue Apr 27 13:18:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: Szmania, Susann M: "[Reagent source?]" <BR/><BR/>Previous message: VSH - Tech Support: "Creating a database of FCS files" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Wed Apr 28 2004 - 03:12:04 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-3817221324782635702008-10-14T22:47:00.000-05:002008-10-14T22:47:00.000-05:00ISAC Montpellier CyberCafeThis message: [ Message ...ISAC Montpellier CyberCafe<BR/><BR/>This message: [ Message body ] [ More options ] <BR/><BR/>Related messages: [ Next message ] [ Previous message ] <BR/><BR/>From: Adam Treister adam@treestar.com<BR/><BR/>Date: Tue May 11 2004 - 17:28:22 EST<BR/><BR/>Dear ISAC Montpellier attendees:<BR/><BR/>We once will again be putting on an Internet cafe for your<BR/><BR/>gastronomical and connectivity pleasure at ISAC.<BR/><BR/>This year, we'll be rechristening the establishment. Its has been<BR/><BR/>renamed the Fluor-de-lis. See our logo, below. We've adopted the<BR/><BR/>twelfth century crest of King Louis VII, but we've added more colors,<BR/><BR/>and installed a two-way sort. We hope to provide an oasis for you to<BR/><BR/>rest your feet and mind, check in with home, and enjoy an atmosphere of<BR/><BR/>old world provincial charm, combined with blindingly fast packet<BR/><BR/>throughput.<BR/><BR/>Since every time we do this, it gets bigger and more elaborate (on a<BR/><BR/>log scale), we've invited a couple of new companies in to bring you<BR/><BR/>more space, more computers, more food and drink. We're pleased to<BR/><BR/>share the sponsorship of the Fluor-de-lis with two young companies,<BR/><BR/>Biotrue (www.biotrue.net) & Cytopeia (www.cytopeia.com).<BR/><BR/>Both are introducing exciting new products for cytometry. We think<BR/><BR/>you ought to know about them, and they've agreed to donate their<BR/><BR/>boothspace to be your connection to the outside world. ISAC is<BR/><BR/>generously supplying the bandwidth.<BR/><BR/>We'll also be showing brand new versions of FlowJo, for both<BR/><BR/>Mac and Windows. This will be our biggest release ever. If you've <BR/><BR/>heard<BR/><BR/>FlowJo is the flow cytometry software to beat, we're about to raise the<BR/><BR/>bar once again.<BR/><BR/>Au revoir,<BR/><BR/>Adam<BR/><BR/>---------------------------------------------<BR/><BR/>Adam Treister<BR/><BR/>Tree Star, Inc.<BR/><BR/>340 A St.<BR/><BR/>Ashland OR 97520<BR/><BR/>1-800-366-6045<BR/><BR/>---------------------------------------------<BR/><BR/>Received on Wed May 12 16:38:00 2004 <BR/><BR/>This message: [ Message body ] <BR/><BR/>Next message: Robert C. Leif: "BiOS Meeting Announcement" <BR/><BR/>Previous message: Lacey, Simon: "Long wavelength dyes for in vivo staining?" <BR/><BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ] [ By messages with attachments ] <BR/><BR/>This archive was generated by hypermail 2.1.8 : Thu May 13 2004 - 03:12:03 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-54442117752576628202008-10-14T22:43:00.000-05:002008-10-14T22:43:00.000-05:00ISAC Congress Executive Joanne Lannigan joannelan...ISAC Congress Executive Joanne Lannigan <BR/><BR/> <BR/><BR/>joannelanni.._at_virginia.edu with questions <BR/><BR/>University of Virginia's Site License for FlowJo <BR/><BR/>http://www.healthsystem.virginia.edu/internet/cyto ... <BR/><BR/>FlowJo Site License <BR/>Registration Form <BR/><BR/>Complete the form below to access the University of Virginia's Site <BR/>License for FlowJo data analysis software. All fields with * must be <BR/>completed or your registration will not be processed. Once your <BR/>information has been received and verified you will receive a serial <BR/>number which you must enter in FlowJo on the computer whose hardware <BR/>address you provided. Cost per user license will be determined at the <BR/>end of the year depending on the average number of registered users <BR/>for the year. (See instructions for pricing structure) This amount <BR/>will be billed to the PTAO provided below, so please make sure the <BR/>PTAO you provide does not expire before that time. Completion of this <BR/>registration form is a valid request for services and assumes the <BR/>approval of the Principal Investigator of the PTAO account provided. <BR/>Cont <BR/>act <BR/>Joanne Lannigan joannelanni..._at_virginia.edu with questions. <BR/><BR/>*****************************************************************************************************<BR/><BR/> <BR/><BR/> <BR/><BR/>GLIIFCA 2007-2008 Officers and Committees <BR/><BR/>President and Scientific Program Chair Paul Wallace <BR/><BR/>Program Committee Members Mike Sramkoski, Vera Donnenberg <BR/><BR/>Executive Secretary <BR/>Co-secretary <BR/><BR/>Mary Paniagua <BR/>Ryan Duggan* <BR/>Treasurer Brian K. DuChateau <BR/>Education and CMLE Chairs Jonni Moore*********** <BR/>Vendor Liasons: Karen Domenico, Tom Sawyer <BR/>Website Manager Tim Bushnell* *******************<BR/><BR/>STEERING COMMITTEE MEMBERS <BR/>Canada David Hedley , Betty-Anne McBey,Nicole McFarlane <BR/>Illinois Charles Goolsby , Maurice R. G. O'Gorman , Mary <BR/>Paniagua, <BR/>Ryan Duggan* <BR/>Indiana Lisa Green ,** Bartek Rajwa******************** <BR/>Iowa Bruce Pesch , Kristi Harkins <BR/>Massachusetts Betsy Ohlsson-Wilhelm, Brian K. DuChateau <BR/>Michigan Alexander Nakeff , Louis King <BR/>Minnesota Paul Champoux <BR/>New York Paul Wallace , Timothy Bushnell************************ <BR/>Ohio Karen Domenico , Tom Sawyer , Mike Sramkoski <BR/>Pennsylvania Jonni Moore *, Vera Donnenberg <BR/>Virginia Joanne Lannigan************************************** <BR/>Wisconsin Kathy Schell , Kathy A. Muirhead , Matt Hanson <BR/>Emeritus Carleton C. Stewart, Sigrid Stewart <BR/><BR/>Review the Executive Members on ISAC Congresss <BR/><BR/>Re: clinical flow cytometry <BR/><BR/><BR/>This message: [ Message body ] [ More options ] <BR/>Related messages: [ Next message ] [ Previous message ] [ In reply <BR/>to ] [ Next in thread ] <BR/>From: Ryan Farmer far...@treestar.com *********** WEBMASTER<BR/><BR/><BR/>Date: Mon Apr 14 2008 - 19:05:51 EDT <BR/><BR/>There is another site of interest along these lines. <BR/><BR/> Mycyte.org <BR/><BR/> is well established and has a variety of online texts, articles and <BR/>other links (protocols, standards, etc.). Users can expand the <BR/>variety <BR/>and <BR/>amount of information as well. <BR/><BR/>It is located at http://www.mycyte.org. <BR/><BR/>Ryan F ********WebMaster Mycyte.orgAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-87668214203609580482008-10-14T22:40:00.000-05:002008-10-14T22:40:00.000-05:00This archive was generated by hypermail 2.1.6 : Th...This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - <BR/>17:35:29 EST <BR/><BR/>Purdue Cytometry Mailing List: Re: Thanks for the suggestions - <BR/><BR/>[Disclaimer: Yes, I live off FlowJo sales, and that's a blatantly <BR/>commercial statement,<BR/><BR/> but it gets technical from here on.] We've <BR/>played with spatial 3D ... <BR/>www.cyto.purdue.edu/hmarchiv/2006/0939.htm <BR/><BR/>Tim Bushnell & Ryan Duggan ..... Purdue <BR/>University Cytometry Laboratories, West Lafayette, IN ... <BR/>www.gliifca.org/pdf/GLIIFCA-2004.pdf - Similar pages - Note this <BR/><BR/>MyCyte <BR/><BR/>Thanks Tim Timothy Bushnell, Ph.D. Research Assistant <BR/>Professor, ... Justin, We're working hard to finalize FlowJo <BR/>Collectors' Edition, ... <BR/>mycyte.org/index.php? <BR/><BR/>option=com_newsfeeds&task=view&feedid=11&Itemid=70 - 133k - Cached - <BR/>Similar pages - Note this <BR/><BR/>From: Adrian Smith A.Sm...@centenary.usyd.edu.AU <BR/><BR/><BR/><BR/>(Disclaimer: - I provided a some input into the way the platform <BR/>works and I have been beta testing FlowJo for a while now) <BR/><BR/><BR/><BR/>ISAC Congress Bilologiacl Councilor, UR, Webmaster and Great lakes <BR/>Imaging <BR/><BR/>Sales Rep? <BR/><BR/>Subject: Final Call for Flowjo order <BR/>From: "Bushnell, Timothy" tim_bushn...@URMC.ROCHESTER.EDU <BR/>Reply-To: Bushnell, Timothy <BR/>Date: Wed, 13 Aug 2008 16:59:51 -0400 <BR/>Content-Type: multipart/alternative <BR/>Parts/Attachments: text/plain (39 lines) , text/html (40 lines) <BR/><BR/>Content-Type: text/html <BR/>This is the final call for the bulk Flowjo order. I need all orders <BR/>in by Monday afternoon (8/18). The original message is below: <BR/>------------ <BR/><BR/>Hello all: <BR/><BR/>In case you missed our spring order, I am putting together another <BR/>order for Flowjo software. I will be placing it in the month of <BR/>August, to try to take advantage of the<BR/><BR/>“Buy 2- get 1 free” sale. <BR/><BR/>Currently a single dongle costs $1495. <BR/><BR/><BR/>However, if there are three <BR/>investigators interested in getting a dongle during this promotion, <BR/><BR/><BR/>each dongle would cost $997 <BR/><BR/><BR/><BR/>(2x1495 = 2990/3 = $997). <BR/><BR/><BR/>This is a <BR/>great <BR/>price. <BR/><BR/>There is also no limit to the number of free dongles <BR/>available. <BR/><BR/>So, if you are interested, please email me the following information: <BR/><BR/>PI: <BR/>Contact name and number: <BR/>Account number to charge: <BR/><BR/>Regards <BR/>Tim Bushnell <BR/><BR/>-- <BR/>Tim Bushnell, Ph.D. <BR/>Co-Director, URMC Flow Cytometry Facility <BR/>Office: 585-273-5535 <BR/>Cell: 585-690-5157 <BR/>http://www.urmc.rochester.edu/flow-core/ <BR/>http://www.urmc.rochester.edu/wnyfug/ <BR/><BR/>____UR_Cytometry______Subscribers Corner_____ https://lists.rochester.edu <BR/>LISTS.ROCHESTER.EDU ( UR_CYTOMETRY: 24 matches.. ) <BR/><BR/>Item # Date Time Lines Subject <BR/>000139 2008-08-13 16:59 112 Final Call for Flowjo order <BR/>000138 2008-08-06 14:54 75 Summer Flowjo special <BR/>000131 2008-07-17 08:49 104 Re: Reminder: Flowjo lecture and support <BR/>000129 2008-07-17 06:24 73 Reminder: Flowjo lecture and support <BR/>000127 2008-07-14 14:05 87 Tree Star Flowjo Training/discussions, <BR/>Thursday, July 17th <BR/>000081 2008-03-14 12:21 228 Reminder: Spring Flowjo order <BR/>000079 2008-03-03 13:03 221 Spring Flowjo order <BR/>000070 2008-01-23 13:24 291 Feedback requested on Flowjo web seminars <BR/>000069 2008-01-18 09:57 249 Reminder: TODAY 1 pm - Flowjo Seminar on <BR/>DNA Analysis and Cell Proliferation <BR/>000068 2008-01-16 11:31 378 Resend: Reminder: Friday 1 pm - Flowjo <BR/>Seminar on DNA Analysis and Cell Proliferation <BR/>000067 2008-01-16 09:59 220 Reminder: Friday 1 pm - Flowjo Seminar on <BR/>DNA Analysis and Cell Proliferation <BR/>000066 2008-01-11 10:31 221 Reminder: Today 1 pm - Flowjo Seminar on <BR/>Compensation and Transformation <BR/>000065 2008-01-09 11:20 214 Reminder: Friday 1 pm - Flowjo Seminar on <BR/>Compensation and Transformation <BR/>000063 2008-01-04 10:12 193 Reminder: 1 pm - Flowjo Seminar <BR/>000061 2008-01-02 09:46 244 January is Flowjo month at URMC <BR/>000057 2007-12-04 09:13 267 January is FlowJo Month at URMC <BR/>000047 2007-10-19 10:11 209 Today is the last day to join the Flowjo <BR/>bulk order <BR/>000046 2007-10-17 10:55 284 Last call - Fall Flowjo order <BR/>000042 2007-10-09 09:29 256 2nd call - Fall Flowjo order <BR/>000040 2007-10-01 09:30 242 Fall Flowjo order <BR/>000034 2007-07-12 06:35 198 Reminder: Flowjo seminar today 1-3 pm <BR/>000032 2007-07-03 15:36 88 Post WNYFUG 2007 FlowJo Seminar <BR/>000005 2007-04-12 09:41 218 Reminder - Flowjo order <BR/>000002 2007-04-03 14:55 248 Flowjo purchase 2007 <BR/><BR/>In the study of economics and market competition, collusion takes <BR/>place within an industry when rival companies cooperate for their <BR/>mutual benefit. Collusion most often takes place within the market <BR/>form of oligopoly, where the decision of a few firms to collude can <BR/>significantly impact the market as a whole. Cartels are a special <BR/>case <BR/>of explicit collusion. Collusion which is not overt, on the other <BR/>hand, is known as tacit collusion. <BR/><BR/>*********************************************************************************************<BR/><BR/>Answer: The Purdue Cytometry discussion group is strictly for <BR/>discussion of scientific issues relating to the fields in which ISAC <BR/>is generally involved. This includes flow cytomery and imaging and <BR/>basic science areas of cellular function, cell cycling, immunology, <BR/>microbiology and the many areas where single cells or tissues are <BR/>analysed. <BR/><BR/>To join you must send a request to: Subscr...@flowcyt.cyto.purdue.edu <BR/>and you must include your name, your institution and your area of <BR/>scientific interest. All subscriptions are reviewed - it is not <BR/>automatic. <BR/><BR/>**********************************************************************************************<BR/><BR/>> From: J. Paul Robinson <BR/>> Reply To: j...@flowcyt.cyto.purdue.edu <BR/>> Sent: Friday, August 20, 2004 2:25 PM <BR/>> To: Cytometry Mailing List <BR/>> Subject: Re: mr on Apple web site <BR/><BR/>> ummm....Mario says.. <BR/>> "In life sciences - particularly in research life sciences - <BR/>> probably 50 to 70% of research laboratories used <BR/>> Macs"....while I have a passionate dislike for Windows......is <BR/>> this really true ??? or is the key word there "used"?? (Ok...I <BR/>> have put on my helmet and armor....waiting...) <BR/>> paul <BR/><BR/>> > For all of the mr groupies out there in cytometry cyberspace. Don't wet <BR/>> your <BR/>> > pocket protectors over this. <BR/><BR/>> > Honestly though, well deserved praise for Mario & the Tree Star group: <BR/>> > http://www.apple.com/science/profiles/roederer <BR/>**********************************************************************************************<BR/><BR/> <BR/><BR/>Re: mr on Apple web siteAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-77646011057002264032008-10-14T22:31:00.000-05:002008-10-14T22:31:00.000-05:00From: Bushnell, Timothy Timothy_Bushn...@URMC.Roch...From: Bushnell, Timothy Timothy_Bushn...@URMC.Rochester.edu <BR/>Date: Fri Mar 21 2008 - 07:21:57 EDT <BR/><BR/>Colleagues: <BR/><BR/>As you probably know, ISAC is holding elections this year. The <BR/>deadline <BR/>for voting is next Wednesday, March 26th. This was announced via <BR/>email <BR/>and on the ISAC homepage at: <BR/>http://www.isac-net.org/content/view/730/2/ . <BR/><BR/>I am a candidate for Biological Councilor, and would like to share <BR/>with <BR/>you my thoughts on my goals and plans should I be elected. I would <BR/>welcome anyone's comments on these goals because, as Bob pointed out, <BR/>with discussion we can refine our positions and be responsive to you, <BR/>the members of ISAC. <BR/><BR/>What I hope to bring to ISAC is an expansion of the existing programs <BR/>which serve to unite and coordinate existing affiliated societies in <BR/>North America and abroad. ISAC is uniquely positioned to support the <BR/>growth and development of regional meetings and other events to <BR/>facilitate professional networking and contacts directly related to <BR/>cytometry. Networking at such meetings is a very effective way of <BR/>communicating and sharing pertinent information about cytometry - <BR/>often <BR/>to those not affiliated with ISAC - which will increase membership as <BR/>the benefits of ISAC association are presented to an untapped market. <BR/><BR/>These meetings can also serve as a clearinghouse to discuss and <BR/>promulgate the ISAC initiatives including: education, data <BR/>presentation, <BR/>data file standardization, and biosafety. Getting this information in <BR/>the hands of the casual user will greatly enhance ISAC's efforts to <BR/>affect wholesale change in the scientific community. <BR/><BR/>I'm very enthusiastic about adding my efforts to the ISAC - most <BR/>specifically areas I have expertise in: <BR/><BR/>* Expanding the membership base in <BR/>North <BR/>America and abroad by developing ISAC initiated outreach programs, at <BR/>the local and regional level. These programs will seek to identify <BR/>the <BR/>casual user of cytometry, and provide them with the methods, <BR/>protocols <BR/>and standards that ISAC has and will continue to develop. <BR/><BR/>* <BR/><BR/>* Finding, recognizing and recruiting <BR/>cytometry facility core directors as a group vital to our membership <BR/>and <BR/>supporting them by offering ISAC models, standards, information, as <BR/>well <BR/>as an international resource to which they can come with problems, <BR/>questions and issues. <BR/><BR/>* <BR/><BR/>* Supporting and building more <BR/>regional <BR/>meetings with a greater ISAC presence via an information kiosk, <BR/>financial support for speakers, and a level of professional <BR/>camaraderie <BR/>that has been the hallmark of cytometry users around the world. <BR/><BR/>* <BR/><BR/>* Building stronger, mutually <BR/>beneficial <BR/>alliances with corporate sponsors at a regional level to facilitate a <BR/>growing base of new and existing regional groups. <BR/><BR/>My experience in developing and growing the WNYFUG from nothing, as <BR/>well <BR/>as my training as a member and program chair of the long-running <BR/>GLIIFCA <BR/>meeting provides me the perspective and skill-set necessary to <BR/>achieve <BR/>these goals. <BR/><BR/>I am an avid supporter of technology, specifically cytometry based <BR/>instrumentation, for the greater understanding of biology across the <BR/>board - however, with such change and development as we've seen in <BR/>just <BR/>20 years, that needs to be tempered with sound practices, strength of <BR/>information, networking on a global scale so that we are keenly aware <BR/>of <BR/>both developments in cytometry as well as potential pitfalls. The key <BR/>to <BR/>this level of awareness on a global scale is by seeing and serving <BR/>the <BR/>regional areas, where those 'in the trenches' can find guidance and <BR/>direction from those around the world. <BR/><BR/>I would urge all ISAC members to take a few minutes to review the <BR/>candidates and cast a vote. Your selection helps shape the future of <BR/>the Society. <BR/><BR/>Thank you in advance for your support, <BR/><BR/>Tim Bushnell <BR/><BR/>Timothy Bushnell, Ph.D. <BR/><BR/>Research Assistant Professor, Pediatrics and Oncology <BR/><BR/>Co-Director, URMC Flow Cytometry Facility <BR/><BR/>Office: 585-273-5535 <BR/><BR/>Lab: 585-273-1361 <BR/><BR/>Cell: 585-690-5157 <BR/><BR/>Fax: 585-276-0233 <BR/><BR/>http://www.urmc.rochester.edu/Aab/geneped/flow/ <BR/><BR/>http://www.urmc.rochester.edu/wnyfug/ <BR/><BR/>Received on Fri Mar 21 15:58:00 2008Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-52848879333544640502008-10-14T22:28:00.000-05:002008-10-14T22:28:00.000-05:00Re: EMAIL ABUSE - how tostop**********************...Re: EMAIL ABUSE - how to<BR/>stop**************************************************************************<BR/>From: Adam Treister (a...@treestar.com)<BR/>Date: Mon Dec 16 2002 - 16:00:07 EST<BR/>Next message: PAUL HALLBERG: "Summary: Sorting CHO cells"<BR/>Previous message: Mojgan Shaiegan: "RBC phenotyping by flow"<BR/>In reply to: J.Paul Robinson: "EMAIL ABUSE - how to stop"<BR/>Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]<BR/>[ attachment ]<BR/>---------------------------------------------------------------------------<BR/>¬-----<BR/>On Thursday, December 12, 2002, at 05:43 AM, J.Paul Robinson<BR/>wrote:****************************<BR/><BR/><BR/>Colleagues: I am sending out a copy of a message I have just sent to<BR/>RNWAY laboratories of South Korea and all 20 worldwide distributors of<BR/>RNWAY products most of whom are highly reputable companies. I am only<BR/>sending it to you because I am going to propose to create a small<BR/>"SCIENTISTS against EMAIL ABUSE" type of revolutionary action.........<BR/><BR/><BR/>Paul,<BR/>Sounds like you're advocating fighting disease by eradicating the<BR/>antigen instead of boosting the immune response. You can organize<BR/>all<BR/>you want on eliminating the pest, but until they put a stamp tax on<BR/>email, a better approach is to let the<BR/>messages be out there, but have them filtered to oblivion before you<BR/>ever see them.<BR/>In your case, the postmaster at Purdue is probably already filtering<BR/>millions of messages a day that come to the thousands of email users<BR/>on<BR/>campus. They are probably capable of shutting down any RNWAY<BR/>mail,****************************<BR/>and<BR/>spreading the word to other postmasters that they also should filter<BR/>those messages.<BR/>So if you can get the IT people at the university to tighten their<BR/>sieve, that's best. Otherwise you have to switch to an email program<BR/>that has good junk filters. I think I get 500+ messages a day, and<BR/>only 10 to 20 make it past the junk<BR/>filter.<BR/>****************************<BR/>Until last summer I was using Outlook Express and spam was a huge<BR/>problem. Since then I switched to the free Mail program in OS X,<BR/>which<BR/>just added special features for spam detection and removal. Its<BR/>probably 97% effective, and I haven't found any false positives.<BR/>So, of course, the best answer is to get a Mac :)<BR/>I'm sure the PC mail clients are addressing this issue as well. I<BR/>believe there are central databases of offenders so programs can<BR/>learn<BR/>from others which messages to delete. I would imagine this is the<BR/>most important feature in any email program sold these days, so I bet<BR/>Eudora or other third party mail programs have this solved.<BR/>There's a lot of information on the subject at:<BR/>http://spam.abuse.net/<BR/>Whether you fight the problem on the server or the client, it<BR/>definitely is worth getting it cleaned up. I found it screwed up my<BR/>whole communications process because every time I wanted check email,<BR/>I<BR/>had to wade through dozens or hundreds of useless ads.<BR/>Adam<BR/>---------------------------------------------------<BR/>Adam<BR/>Treister<BR/>***********************************<BR/>a...@treestar.com<BR/>www.flowjo.com 800-366-6045Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-70135064474781259662008-10-14T22:17:00.000-05:002008-10-14T22:17:00.000-05:00Collusion is an agreement, usually secretive, whic...Collusion is an agreement, usually secretive, which occurs between <BR/>two <BR/>or more persons to deceive, mislead, or defraud others of their legal <BR/>rights, or to obtain an objective forbidden by law typically <BR/>involving <BR/>fraud or gaining an unfair advantage. It can involve "wage fixing, <BR/>kickbacks, or misrepresenting the independence of the relationship <BR/>between the colluding parties."[1] All acts affected by collusion are <BR/>considered void.[2] <BR/><BR/>In the study of economics and market competition, collusion takes <BR/>place within an industry when rival companies cooperate for their <BR/>mutual benefit. Collusion most often takes place within the market <BR/>form of oligopoly, where the decision of a few firms to collude can <BR/>significantly impact the market as a whole. Cartels are a special <BR/>case <BR/>of explicit collusion. Collusion which is not overt, on the other <BR/>hand, is known as tacit collusion. <BR/><BR/>According to neoclassical price-determination theory and game theory, <BR/>the independence of suppliers forces prices to their minimum, <BR/>increasing efficiency and decreasing the price determining ability of <BR/>each individual firm. However, if firms collude to increase prices <BR/>loss of sales is minimized as consumers lack alternative choices at <BR/>lower prices. This benefits the colluding firms at the cost of <BR/>efficiency to society. <BR/><BR/>One variation of this traditional theory is the theory of kinked <BR/>demand. Firms face a kinked demand curve if, when one firm decreases <BR/>its price, other firms will follow suit in order to maintain sales, <BR/>and when one firm increases its price, its rivals are unlikely to <BR/>follow, as they would lose the sales' gains that they would otherwise <BR/>get by holding prices at the previous level. Kinked demand <BR/>potentially <BR/>fosters supra-competitive prices because any one firm would receive a <BR/>reduced benefit from cutting price, as opposed to the benefits <BR/>accruing under neoclassical theory and certain game theoretic models <BR/>such as Bertrand competition. <BR/><BR/>Practices that facilitate tacit collusion include: <BR/><BR/>Uniform prices <BR/><BR/>A penalty for price discounts <BR/><BR/>Advance notice of price changes ************************** <BR/><BR/>Information exchange ****************** <BR/><BR/>From: Adam Treister (a...@treestar.com) <BR/>Date: Mon Apr 22 2002 - 00:15:07 EST <BR/>* Reply: Mario Roederer: "Job Opening -- Immediate -- Vaccine <BR/><BR/>______________________________________... <BR/>Only two more weeks until ISAC, that biennial bacchanalia of flower <BR/>power <BR/>and fun! So I hope you'll excuse a bunch of blatantly commercial <BR/>announcements to the list, but endulge me this one time and read on. <BR/><BR/>At the show we'll be releasing FlowJo Version 4. We've got new <BR/>platforms <BR/>for overlaying and clustering, we've made it work better across the <BR/>Internet, added all sorts of new <BR/><BR/>For those who haven't found it yet, we've unveiled a spanking new <BR/>FlowJo <BR/>website. Flowjo.com is chuck full of new content, functionality and <BR/>spunk. <BR/>Automated price quotes, online ordering, a FAQ that will guide you to <BR/>new <BR/>depths of understanding, and none of that awful yellow on black text. <BR/>The <BR/>search engine even works. No ads & cookie-free. Check it out. <BR/>************* <BR/>Specifically, you should check our pricing. Prices are going up on <BR/>******* <BR/>May 10. ************ <BR/>It has been a number of years since we've changed our prices and with <BR/>the <BR/>development of the OSX and PC versions, it¹s time for a leap. I <BR/>guess <BR/>there's no such thing as a free launch. Anyway, this may be a great <BR/>time to <BR/>buy that FlowJo ten pack you've been thinking about. Licenses <BR/>purchased <BR/>before May 10 are entitled to a year of free upgrades, including the <BR/>4.0 <BR/>release. <BR/>Unleash the flower power! <BR/>Adam <BR/>-- <BR/>Adam Treister <BR/>Tree Star, <BR/><BR/>Recent FlowJo announcement<BR/><BR/>From: Steve Kelley <BR/>(SKEL...@flowcyt.cyto.purdue.edu) <BR/>Date: Wed Dec 30 1998 - 06:13:04 EST <BR/><BR/>Next message: Steve Kelley: "Possible minor disruption" <BR/>Previous message: Mark A. Corio: "Chemdex no help..." <BR/>Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] <BR/>[ attachment ] <BR/>I know that many people on the mailing list are adamantly opposed to <BR/>anything that even looks a little commercial, and to try to forestall <BR/>any possible <BR/><BR/>complaints about the FlowJo announcement <BR/>, I'll explain what <BR/><BR/>I'd like to see. In my opinion, announcements about new products, and <BR/>product updates are completely appropriate as long as they aren't <BR/>abused. <BR/> I've never wanted to specifically encourage that, because I really <BR/>don't want to be put into a position of having to decide whether a <BR/>message is an <BR/><BR/>"announcement" <BR/>or an <BR/><BR/>"advertisement" <BR/>. The companies involved in our cytometry community have always been <BR/>extremely good 'citizens' as far as I can tell. <BR/><BR/>We have representatives of many companies on the list, and they have <BR/>always had the power to make my life miserable, and the list no more <BR/>than junk mail. Instead, they have helped build this mailing list <BR/>into one of the most useful around, through their contributions and <BR/>responses. I'm not going to try to set specific rules about what <BR/>people can say about their own products, and when they can say it. <BR/>I'll just ask that everyone continue to show restraint; that the <BR/>commercial representatives ask themselves before they submit an <BR/>announcement whether they'd mind seeing every other company in the <BR/>business sending the same message they are about to, and that the <BR/>non- <BR/>commercial (and anti-commercial) people accept discreet announcements <BR/>as simple information, and continue the sometimes brisk discussions <BR/>about problems and benefits of particular products, alongside the <BR/>purely scientific (and occasionally purely entertaining) <BR/>conversation. <BR/>Steve Steve KelleyAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-17719211811272547752008-10-14T22:15:00.000-05:002008-10-14T22:15:00.000-05:00ISAC Homepage - ISAC E-News -- March 2008 Mar 13, ...ISAC Homepage - ISAC E-News -- March 2008 <BR/>Mar 13, 2008 ... Since the Society’s inception, we have developed a <BR/>mailing list which .... Mark Munson, Verity Software House, Inc., E- <BR/>mail: sa...@vsh.com. ... <BR/>www.isac-net.org/content/view/732/44/ - 53k - Cached - Similar pages <BR/>- <BR/>Note this <BR/><BR/>The Daily Dongle <BR/>Cytometry Developers Workshop is attended by the industry and academia <BR/>alike. ... J Paul Robinson delivered an effective summary of the <BR/>problem, ... <BR/>flowjo.typepad.com/ - 40k - Cached - Similar pages - Note this ... <BR/><BR/> <BR/><BR/>Joseph Robinson: ZoomInfo Business People Information <BR/>... Paul Robinson, chair of the Optics East Life Sciences symposium **********<BR/>(see sidebar) and director of Purdue University Cytometry Laboratories <BR/>(West Lafayette, ... <BR/>www.zoominfo.com/people/Robinson_J._-82453.aspx - 24k -<BR/><BR/> <BR/><BR/>International Congress Montpellier 2004 <BR/>File Format: PDF/Adobe Acrobat - View as HTML <BR/>Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of <BR/>Plants .... analytical cytometry publishing and software companies, <BR/>pharmaceuti- ... <BR/>www.afc.asso.fr/actu/congres/isac04.pdf - Similar pages - Note this <BR/><BR/><BR/><BR/>Africa Business Conference 2007 <BR/>J. Paul Robinson Director, Purdue University Cytometry Laboratories <BR/>and Deputy ... in bioengineering with hardware and software groups <BR/>developing innovative ... <BR/>www.hbsafricaconference.org/2007/panels_healthcare.html - 27k - Cached <BR/>- Similar pages - Note this<BR/><BR/> <BR/><BR/>Review Members and Corporations on the Purdue Cytometry Mial list <BR/><BR/>Photonics: Principles and Practices - Google Books Resultby Abdul Al- <BR/><BR/>Azzawi - 2006 - Science - 968 pages <BR/>Newport Corporation. Optics and mechanics. Newport 1999/2000 <BR/>catalog, ... Robinson. Paul, Laboratory Manual to Accompany <BR/>Conceptual <BR/>Phvsics. ... <BR/>books.google.com/books?isbn=0849382904... <BR/><BR/>Purdue Cytometry Mailing List: RE: EPICS C data <BR/>From: J.Paul Robinson <BR/>(j...@flowcyt.cyto.purdue.edu) .. <BR/><BR/>. From: Suzanne Leif President of ********<BR/>Newport Instruments Via Robert C. Leif, Ph.D. Vice President ... <BR/>www.cyto.purdue.edu/hmarchiv/1998/1995.htm - 7k - Cached - Similar <BR/>pages - Note this <BR/>Dayong JinJingli Yuan’s group), <BR/> Newport Instruments **************<BR/>(Dr. Robert Leif’s group) and <BR/>Purdue University Cytometry Labs <BR/> (Prof. J.Paul Robinson’s group). ... ******<BR/><BR/>www.ics.mq.edu.au/gen/person/jin.html - 13k - Cached - Similar pages <BR/>- <BR/>Note this <BR/>Data and Image Analysis Special Interest Group Meeting 2007J. Paul <BR/>Robinson, SVM Professor of Cytomics, Purdue University and <BR/>President, ... (*) Robert C. Leif, Newport Instruments. "Cytometry <BR/>Standards Continuum" ... <BR/>www.ravkin.net/SBS/D&IA_SIG2007.htm - 14k - Cached - Similar pages - <BR/>Note this <BR/><BR/>[PPT] DIA SIG 2007: What are the Issues?File Format: Microsoft <BR/>Powerpoint - View as HTML <BR/>... J. Paul Robinson, President, International Society for Analytical <BR/>Cytology, ... (*) Robert C. Leif, Newport Instruments "Cytometry <BR/>Standards Continuum" ... <BR/>www.ravkin.net/SBS/DIA%20SIG%202007%20Intro.ppt - Similar pages - <BR/>Note <BR/>this <BR/><BR/>Wiley Cytometry - ISAC 2000 International Congress POSTER <BR/>ABSTRACTS <BR/>Stephanie Ann Sincock, <BR/>Purdue University; J.Paul Robinson, <BR/>Purdue University ...... Robert Leif, Newport Instruments; John <BR/>Quagliano, Los Alamos National ... <BR/>www.wiley.com/legacy/products/subject/life/cytometry/isac2000/posters... <BR/>- 223k - Cached - Similar pages - Note this <BR/><BR/>Verity Software House, Inc. <BR/>10 New Lewiston Road <BR/>Topsham, Maine 04086 <BR/>207 729 6767 voice <BR/>207 729 5443 fax <BR/><BR/>Phoenix Flow Systems <BR/>11575 Sorrento Valley Road, Suite 208 <BR/>San Diego, CA 92121 <BR/>619 453 5095 voice <BR/>619 259 5268 fax <BR/><BR/>Cytomation, Inc. <BR/>400 E. Horsetooth Rd., Suite 100 <BR/>Fort Collins, CO 80525 <BR/>800 822 9902 voice <BR/>303 226 2322 fax NEW ADDRESS FORT COLLINS DRIVE <BR/><BR/>DOES THE FEDERAL GOVERNMENT REGULATE ISAC CONGRESS ...<BR/><BR/> <BR/><BR/>A numerical recipe for accurate image reconstruction from discrete ... <BR/>- 2 visits - Nov 28 <BR/>J. Paul Robinson, Bindley Bioscience Center, Purdue University, 1203 <BR/>West State St., West Lafayette, IN 47907, USA and Cytometry <BR/>Laboratories, ... <BR/>portal.acm.org/citation.cfm?id=1221224 - Similar pages - Note this <BR/><BR/>Ø [PDF] <BR/>> GREAT LAKES INTERNATIONAL IMAGING AND FLOW CYTOMETRY <BR/>> ASSOCIATION<BR/>> File Format: PDF/Adobe Acr <BR/>> obat - View as HTML <BR/>> J. Paul Robinson, Philip Marder. Iowa. Bruce Pesch. Michigan .... flow <BR/>> cytometry software. Clearly, traditional cytometers can not handle the <BR/>> demands of HTS ... <BR/>> gliifca.org/pdf/GLIIFCA-2001.pdf - Similar pages - Note this <BR/>> International Congress Montpellier 2004 <BR/>> File Format: PDF/Adobe Acrobat - View as HTML <BR/>> Co-Chairs: Bartek Rajwa and J Paul Robinson. The Cytometry of <BR/>> Plants .... analytical cytometry publishing and software companies, <BR/>> pharmaceuti- ...www.afc.asso.fr/actu/congres/isac04.pdf- Similar pages - Note this<BR/><BR/> <BR/><BR/>Purdue Cytometry Mailing List: RE: CFSE graphs (UNCLASSIFIED) <BR/>... Verity's MODFIT software has a very nice tool for <BR/>proliferation ... Stelekati [mailto:e...@fz-borstel.de] >>>Sent: <BR/>Monday, March 26, 2007 6:58 AM >>>To: Cytometry Mailing List <BR/><BR/>>>>Subject: CFSE graphs >>> >>>Dear all, >>>I want to ... http://flowcyt.cyto.purdue.edu/hmarchiv/Current/0490.htm - 9.0KB <BR/>72% <BR/>|||||||||||||||||||| <BR/>30 Mar 07 <BR/><BR/><BR/><BR/>Purdue Cytometry Mailing List: 31st Annual Flow Cytometry Course ... <BR/>From : Mark - Verity Software House m...@vsh.com ... and lectures <BR/>you'll learn about pertinent topics in the art and science of flow <BR/>cytometry. ... <BR/>www.cyto.purdue.edu/hmarchiv/Current/0455.htm - 8k - Cached - Similar <BR/>pages - Note this <BR/><BR/>Purdue Cytometry Mailing List: ModFit LT 3.1 Service Pack 1 now ... <BR/>For more information, contact Verity at sa...@vsh.com. Best regards, <BR/>Mark Mark E. Munson Sales Manager Verity Software House, Inc. 45A <BR/>Augusta Road PO Box ... <BR/>www.cyto.purdue.edu/hmarchiv/2003/1134.htm - 6k - Cached - Similar <BR/><BR/> <BR/><BR/>From: J. Paul Robinson j...@flowcyt.cyto.purdue.edu<BR/><BR/>Date: Mon Mar 31 2008 - 01:18:05 EDT <BR/><BR/> <BR/><BR/>From J. Paul Robinson - Moderator <BR/><BR/>Robert is right - there is too much politics and not enough <BR/>science... <BR/><BR/>What is happening here, is that there are too many cooks. <BR/><BR/>Let me make it very clear that we work hard to keep this list clean. <BR/>It <BR/>does not always work. When we identify a failure, we usually respond <BR/>to <BR/>the person concerned and don't waste all your time. <BR/><BR/>We frequently note in our posts, that advertising is not allowed. <BR/>This <BR/>list was developed from 2 or 3 individuals who actually had email in <BR/>1990, to 3000 over the past 19 years. It did not happen by chance, <BR/>nor <BR/>was it overnight. It was developed with a lot of cost ($$$), a lot of <BR/>time, and what was a pretty darned good idea when it started. We <BR/>don't <BR/>tolerate people who try to damage the list. <BR/><BR/>Now that it's highly successful, there are a number of individuals <BR/>that <BR/>are trying to either circumvent the list, use it for their own <BR/>purposes, <BR/>or simply sideline it. There are even some proposing that they should <BR/>be <BR/>able to manipulate the list and its contents in any forum for any <BR/>purpose. I am really shocked at this rather callous approach to a <BR/>scientific discussion board. I am not making public those individuals <BR/>or <BR/>their companies, but if I am pushed, I will identify them publicly. <BR/>If <BR/>I <BR/>do so, and they create havoc, it could shut the list down - or end up <BR/>in <BR/>a nasty legal battle. I don't suppose that would be popular? <BR/><BR/>So, it seems to me, that we need to get back to basics and focus on <BR/>the <BR/>reason this list has been so successful (and why you all want to use <BR/>it <BR/>for advertising - or even why those who what to hijack it...) - it <BR/>does <BR/>a good if not great job overall. <BR/><BR/>thanks for your support - all 3000 of you ...well most of you! <BR/><BR/>Clearly we will provide a mechanism for companies to provide a means <BR/>for <BR/>communication....it's on our list... <BR/><BR/>Maybe I am getting too old for all this abuse....! <BR/><BR/>regards <BR/><BR/>Paul Robinson <BR/>Purdue UniversityAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-50565063648514339882008-07-14T23:29:00.000-05:002008-07-14T23:29:00.000-05:00From: Adam Treister a...@treestar.com> Date: Sun J...From: Adam Treister a...@treestar.com<BR/>> Date: Sun Jan 04 2004 - 13:48:24 EST<BR/>> David,<BR/>> We've tried on a couple of occasions to add a "spatial" 3D module to<BR/>> FlowJo, and it has never turned out well enough to make it into a<BR/>> release. We've found that using time as the third dimension, as in<BR/>> our<BR/>> "data movies," or using several 2D graphs, as in our "multi-graph<BR/>> overlay" to be more practical solutions.<BR/>> If you want smoothing or density coloring, that requires binning the<BR/>> data. Even working at low resolution, you're looking at 256 times as<BR/>> much time and memory to take the plot into an additional dimension.<BR/>> You might get a tenfold performance increase with the G5 (which I<BR/>> think<BR/>> is quite optimistic, because the G5 adds fast floating point<BR/>> processing, but binning is a integer operation), but even with that<BR/>> that, adjusting a gate goes from taking perhaps a second to almost a<BR/>> half minute. That would make using FlowJo feel like using CellQuest<BR/>> (just kidding ;) At the full resolution of DiVa files, you're<BR/>> looking<BR/>> at another thousand fold increase over the 2D version, or a billion<BR/>> times (1000 ^ 3) as long as we take to do it now. So, as best I<BR/>> can<BR/>> figure it, we can only support 3D at the cost of losing interactivity<BR/>> with the data (ie, we can make the views, but changing gates or<BR/>> parameters won't immediately change the 3D visualization).<BR/>> It would be tough to have contours in 3D as each layer would obscure<BR/>> the ones inside it. Contours would have to have varying opacity,<BR/>> which<BR/>> not only increases the computational time and complexity, but would<BR/>> make it hard to differentiate populations. And the user interface<BR/>> for<BR/>> gating in space would be a real challenge. You could chop thru space<BR/>> with planes, but that's 1D gating, which doesn't give you more<BR/>> capability to define populations than you have now. So we'd have to<BR/>> invent polyhedral gating.<BR/>> If all you want to do is look at already-gated populations in 3D,<BR/>> there<BR/>> are options that exist. Expo32 has this feature, if you can figure<BR/>> out<BR/>> how to use Beckman Coulter (actually, ACS wrote it) software to view<BR/>> BD<BR/>> files. You can use existing commercial programs (Aabel & JMP are<BR/>> nice<BR/>> Mac 3D applications, and I'm sure there are others), or write it<BR/>> yourself in OpenGL. I think OpenGL would be a better choice than C+<BR/>> +.<BR/>> OpenGL is a higher level graphics language, and knows how to access<BR/>> the<BR/>> specialized accelerators in the graphics cards, which are actually<BR/>> faster for this stuff than the G5. That's how the dungeon games do<BR/>> the<BR/>> 3D shading and rendering.<BR/>> I'll be happy to donate a considerable amount of code that I've done<BR/>> in<BR/>> this effort (most was taken from an old freeware program called<BR/>> Rotator, which I no longer could find with Google, but I have<BR/>> somewhere<BR/>> in my archives), but we decided this was pretty much a dead end.<BR/>> Rotator was in C, and quite unreadable. For the investment this task<BR/>> would take, I think it'd be better to start over in OpenGL. It's<BR/>> cross-platform too, which is important, as you'll find you want to<BR/>> port<BR/>> it to a Cray.<BR/>> I'd still think you want to use FlowJo to read the DiVa files,<BR/>> compensate them, transform them, gate them, and then export desired<BR/>> subpopulations to the 3D viewer. If it were any other instrument,<BR/>> you<BR/>> could probably read the files yourself, with R or our free Java<BR/>> libraries, but the DiVa files are a unique format, and almost always<BR/>> require compensation and transformation to a lin/log scale, so<BR/>> there's<BR/>> a lot of work before you even get to viewing them.<BR/>> I've promised you 3D graphs in FlowJo in the past, and I've done my<BR/>> best to deliver them, but the results have been pretty<BR/>> disappointing.<BR/>> And the benefit of them has never been demonstrated. If you can show<BR/>> us how 3D views provide more interpretable data than our current<BR/>> "compromise solutions," that would help. If you want to pick a<BR/>> data<BR/>> file, we'll make you get a spinning, stereoscopic, 3D view of it. If<BR/>> we find that other scientists are able to make conclusions about the<BR/>> data better than they can from our existing visualizations, that will<BR/>> go a long way towards bumping it up on the FlowJo future feature<BR/>> list.<BR/>> I hope that helps.<BR/>> AdamAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-84892104150463895782008-07-14T23:23:00.000-05:002008-07-14T23:23:00.000-05:00On Thursday, March 16, 2006, at 02:06 AM, A.J. Ro...On Thursday, March 16, 2006, at 02:06 AM, A.J. Rossini wrote: <BR/>> On 3/13/06, Mario Roederer roede..drmr wrote: <BR/>>> I am a firm believer in the use of these transformations--in the <BR/>>> multicolor> world, <BR/>> they have revolutionized our ability to look at the data. (Note that> <BR/>> the transforms in <BR/>> no way affect statistics or gating--they ONLY affect the> visual <BR/>> representation of the <BR/>> data). The point of these transforms is to> make the data more easily <BR/>> conveyed to <BR/>> readers--something that a simple log> scale (which is itself a <BR/>> transformation!) no longer <BR/>> does adequately. <BR/>> I'm going to nitpick a bit -- while the general overall <BR/>> conclusionsMario draws are <BR/>> correct, there are a few subtle points. If you don'tget what I'm <BR/>> saying, believe Mario, <BR/>> it's simpler that way. Read onfor the fine details: <BR/>> Transformations actually do affect the statistical <BR/>> analysis/conclusions. <BR/>> data != transformed data. <BR/>> Conclusions drawn on transformed data do not have to be the same as <BR/>> onraw data (a <BR/>> difference on a log scale using a particular statisticisn't <BR/>> necessarily different using <BR/>> the raw data with the samestatistic). <BR/>> Why transform? <BR/>> To pull the data back to a state of nature where we feel <BR/>> comfortablewith decision making. <BR/>> To pull the data back to a state of nature where certain <BR/>> probabilisticassumptions are <BR/>> met, allowing for "believable" statistical estimationand testing (and <BR/>> opposed to <BR/>> unbelieveable statistical muck) <BR/>> To focus on particular ranges or features of the data. <BR/>> All are valid. <BR/>> The detail is just "comparison on the transformed scale isn't the <BR/>> sameas comparison on <BR/>> the original scale". The point is that it's ratherimportant to do, <BR/>> both to match up <BR/>> with historical scientificapproaches as well as to match up with <BR/>> common practices <BR/>> forcomparability within the cytometric field. <BR/>>>> The Data Presentation Standards Committee, formed by ISAC, will take <BR/>>>> the> <BR/>> responsibility to contact the editors of the Journal with which there <BR/>> are> problems. Our <BR/>> goal is to educate not only the reviewers, but importantly> the <BR/>> Editors--so that they can <BR/>> be made aware that any complaints that> reviewers might have regarding <BR/>> this visualization <BR/>> are baseless. <BR/>> This is important! Visualizations, done well, are critical <BR/>> formultidimensional data. <BR/>> best,-tony <BR/>> (Novartis Pharma AG / University of Washington) <BR/>> blindgl...@gmail.comMuttenz, Switzerland."Commit early,commit often, <BR/>> and commit in a <BR/>> repository from which we can easilyroll-back your mistakes" (AJR, <BR/>> 4Jan05). <BR/><BR/><BR/>Julia Whynot <BR/>Rockefeller University <BR/>Investigative Dermatology <BR/>212-327-7581 <BR/>Received on Mon Mar 20 20:38:00 2006 <BR/>This message: [ Message body ] <BR/>Next message: Moody, Pamela: "Meeting Next Week at Cold Spring Harbor <BR/>Laboratories" <BR/>Previous message: Derek Davies: "UK position" <BR/>In reply to: A.J. Rossini: "Re: Re digital data" <BR/>Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ] <BR/>[ By Author ] [ By messages with attachments ] <BR/>This archive was generated by hypermail 2.1.8 : Tue Mar 21 2006 - <BR/>03:12:02 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-39674685288240010932008-07-14T20:53:00.000-05:002008-07-14T20:53:00.000-05:00For all of the mr groupies out there in cytometry ...For all of the mr groupies out there in cytometry cyberspace. Don't wet your pocket protectors over this. <BR/><BR/>http://www.apple.com/science/profiles/ro... On Aug 20, 2004, at 2:25 PM,<BR/>J. Paul Robinson wrote:<BR/><BR/> ummm....Mario says.. "In life sciences -- particularly in research life sciences -- probably 50 to 70% of research..Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-23171766295778244972008-07-14T20:29:00.000-05:002008-07-14T20:29:00.000-05:00Re: Re digital data This message: [ Message bod...Re: Re digital data<BR/> This message: [ Message body ] [ More options ]<BR/> Related messages: [ Next message ] [ Previous message ] [ In reply<BR/> to ]<BR/> From: WEHICytometry <BR/> Date: Tue Mar 14 2006 - 23:28:16 EST<BR/><BR/> On 14/03/2006, at 9:03 AM, Mario Roederer wrote:<BR/><BR/>> Actually, there aren't several transforms -- basically there are<BR/>> only two ("Logicle", from Dave Parks and Wayne Moore at Stanford,<BR/>> used by DiVa and FlowJo; and the "HyperLog" from Bruce Bagwell at<BR/>> Verity used by WinList). And, in fact, these two are<BR/>> mathematically nearly identical; the Logicle function is a slightly<BR/>> more complex, and slightly smoother version, but probably without<BR/>> noticeable visual difference when the same parameters to the<BR/>> function are used. The primary difference between the two is that<BR/>> the Logicle algorithm provides for a mechanism to automatically<BR/>> select parameters to the function that are optimized to the actual<BR/>> distribution of the data--hence, the transformation can, if<BR/>> desired, be stronger or weaker for one fluorescence channel than<BR/>> for another (which is reasonable, as the magnitude of the error in<BR/>> the measurement is very different in these channels, and the error<BR/>> in the measurement is the primary reason we need the transforms!).<BR/>><BR/><BR/> I feel obliged to correct Mario there; there are indeed more than<BR/> two. Another is the "split scale" form that is used in the WEASEL<BR/> fcm analysis and display program (see http://www.wehi.edu.au/ (Who's computer is this?)<BR/> cytometry/WEASELv2.html). This transform is mathematically simpler<BR/> but, I assert, no less valid. It has been used in WEASEL for a year<BR/> or so and was described to the Australasian Flow Cytometry Group<BR/> last<BR/> year (see the abstract at http://www.wehi.edu.au/cytometry/Abstracts/ (Who's computer is this?)<BR/> AFCG05B.html).<BR/><BR/> Frank.<BR/><BR/> | | << The Cytometry Laboratory<BR/> \__/ <<<< The Walter & Eliza Hall Institute<BR/> ------!!<<<<<< 1G Royal Parade, Parkville<BR/> /!!\ <<<< Victoria 3050, Australia<BR/> o !! \ << ph: 61_3_9345 2540, fax: 61_3_9347 0852<BR/> Received on Wed Mar 15 16:18:00 2006<BR/> This message: [ Message body ]<BR/> Next message: Matt Gordon: "Re: Fixation of cells for TUNEL assay"<BR/> Previous message: Carol Oxford: "LSRII pressurized waste tank"<BR/> In reply to: Mario Roederer: "Re: Re digital data"<BR/> Contemporary messages sorted: [ By Date ] [ By Thread ] [ By Subject ]<BR/> [ By Author ] [ By messages with attachments ]<BR/> This archive was generated by hypermail 2.1.8 : Thu Mar 16 2006 -<BR/> 03:12:01 ESTAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-11951138367038171012008-07-14T18:50:00.000-05:002008-07-14T18:50:00.000-05:00On Thu, 18 Oct 2001, Ray Hicks wrote: some interes...On Thu, 18 Oct 2001, Ray Hicks wrote: <BR/>some interesting points, as did Mario, and although I have not checked <BR/>for typos or glaring grammatical errors, I have snipped them all except this bit <BR/> The other issue I take is; how is the collective going to select the<BR/> experts? Surely the people who are publishing this stuff ARE people "with a<BR/>> modicum of experience in flow". Putting the responsibility on editorial<BR/>> boards is probably going to end up in a status quo. How about pressuring<BR/>> your lab-fellows to sling the FACS aspect of papers, that they're reviewing<BR/>> anyway, in your direction?Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-8037707352330428788.post-86469427978821041212008-07-14T18:40:00.000-05:002008-07-14T18:40:00.000-05:00At 01:00 PM 10/16/01 -0400, Roederer, Mario (VRC) ...At 01:00 PM 10/16/01 -0400, Roederer, Mario (VRC) wrote: <BR/><BR/>>This topic strikes a nerve with many of us. Indeed, ISAC did at one point<BR/>>have the decent notion to have a committee on "data presentation standards"<BR/>>or something like that. I remember seeing something at Montpellier--a<BR/>>pamphlet on presentation, I think. Since then, I haven't heard about the<BR/>>progress of this committee. I made a number of suggestions on the<BR/>>committee's effort, as it was a reasonable start, but don't know if that had<BR/>>any affect. Indeed, even this pamphlet had a number of mistaken notions,<BR/>>showing how ingrained things can get even within the community.<BR/>><BR/>>For example, there was the suggestion that we should always put numbers on<BR/>>the Y axis of a univariate histogram ("# of cells"). In reality, these<BR/>>numbers are meaningless--they depend on the resolution with which the data<BR/>>is binned, which can vary from program to program and instrument to<BR/>>instrument. The reasoning was that the only way to compare histograms was<BR/>>to have these numbers to ensure that the data was interpreted properly.<BR/>>However, this is a misconception--in reality, the peak height in a histogram<BR/>>is rarely meaningful; it is the peak area which carries meaning. What is<BR/>>necessary in a histogram presentation is to identify how many cells were<BR/>>collected (and displayed in the histogram), and, if any peak in the<BR/>>histogram is cut off, to identify what fraction of the vertical scale is<BR/>>shown. I.e., the only thing worth putting on the Y axis label is "% max",<BR/>>where "max" is the maximum peak height. Admittedly, many of my papers have<BR/>>the meaningless numbers on the axis... but I'm still learning...<BR/>><BR/>>I am sure that even this little discussion may set off a minor<BR/>>firestorm--and that's probably good: it will be educational, which is the<BR/>>main point of this list! (By the way, remember that contour plots are also<BR/>>histograms (2D histograms), and they have no numbers on the "Z" axis<BR/>>corresponding to event frequency. Why should univariate histograms have<BR/>>them?)<BR/>><BR/>>Jim Houston asks about the needed information for histograms or dot<BR/>>plots--always, the minimum information is the number of events displayed.<BR/>>(And yes, I am guilty of not always putting that information in my own<BR/>>publications.) I still strongly advocate against dot plots; there are much<BR/>>more informative displays available.<BR/>><BR/>>But the point of this email is not to address the specific defects in data<BR/>>presentation, nor even to start to lay them out. That, in fact, would be<BR/>>better done in a book.<BR/>><BR/>>Both Jim and Robert Zucker bring up the lack of the Community's involvement<BR/>>in peer review. It is worth noting that JAMA requires every paper to be<BR/>>reviewed by a statistician, outside of the normal review. Why not have the<BR/>>same thing for every flow paper? It seems that the major publications<BR/>>should require an expert to review papers containing FACS<BR/>>presentations/analyses for appropriateness. But it won't happen: if we<BR/>>can't even police our own Journals to ensure appropriate data presentation,<BR/>>then what makes anyone think we have the competence to do so for other<BR/>>Journals?<BR/>><BR/>>Some years ago, a few of us bantied around an idea of "post-publication"<BR/>>review of articles that would be placed online. The concept was as follows:<BR/>>each major journal would be assigned to one or two expert reviewers. Each<BR/>>issue would be examined for articles that had flow cytometry in them, and<BR/>>then the reviewer would go over the paper with a predefined list of<BR/>>criteria. The review would explicitly avoid any judgment about the paper's<BR/>>conclusions; it would only address whether the flow cytometric analyses were<BR/>>properly presented, interpreted, and then to note what additional<BR/>>information is required, what possible artifacts need to be eliminated, etc.<BR/>>The review process would be fundamentally based on a checklist (e.g., "was<BR/>>cell viability assessed?", "what staining controls were performed?", "is the<BR/>>data properly compensated?", "did the authors note how many events were<BR/>>displayed?", "are the statistical intreprations of low event counts<BR/>>appropriate?" etc. etc.... I could envision a 100-item list). There would<BR/>>be "sub-lists" for different types of flow, like "cell cycle",<BR/>>"immunophenotyping", "intracellular detection", and "it's obvious I dropped<BR/>>my samples off at my local core facility, didn't tell them what was in each<BR/>>tube, forgot my controls anway, had them generate a few graphs for me, and<BR/>>then xeroxed them until the dots I didn't like went away, so don't blame me<BR/>>because I can't understand the difference between a contour plot and a<BR/>>photomultiplier tube." The reviews would be posted on-line.<BR/>><BR/>>The idea of the online post-publication review is that the general<BR/>>scientific community, when reviewing an article, could turn to the web site<BR/>>and quickly see if there are major problems with the technology that they<BR/>>might not appreciate because of the subtleties. Since the criteria would<BR/>>all be published online as well, the goal would be that authors would start<BR/>>turning to this site before publication in order to better present data,<BR/>>rather than seeing criticisms of their papers show up afterwards. Authors<BR/>>might be allowed to appeal aspects of a review that they feel are<BR/>>inappropriate, thereby providing an ongoing evolution of the evaluation<BR/>>process. There might even be a manuscript pre-review service where authors<BR/>>could ensure appropriateness before submitting for review.<BR/>><BR/>>What would this require? No more than a one or two dozen FACS-savvy people<BR/>>to volunteer for this public service. Anyone with a modicum of experience in<BR/>>flow would be excellent for this; in fact, it's probably better to recruit<BR/>>younger (less jaundiced) people for the process. In reality, the review<BR/>>process would be very rapid, since these are not detailed reviews aimed at<BR/>>the science of the paper, but only at the data presentation. I was so hot<BR/>>on this idea (now 2 years old) that I even registered a domain for its use<BR/>>(http://www.sciwatch.org)--a registration I renew in the hopes that<BR/>>something might actually come of it.<BR/>><BR/>>In my idealistic vision, eventually journals would turn to the Flow<BR/>>community to do this as a standard of practice rather than have it go on<BR/>>post-publication. Journals might even adopt the standard data presentation<BR/>>requirements. People might actually publish FACS data that we can believe.<BR/>><BR/>>But maybe we need to start at home first. I'd like to suggest that<BR/>>Cytometry and Clinical Communications in Cytometry both make an editorial<BR/>>decision to require all published papers to come up to some minimum<BR/>>acceptable standard. If these journals make the commitment, then perhaps<BR/>>there will be enough motivation for a document outlining these procedures to<BR/>>be put together. However much it makes sense, I do not suggest that this be<BR/>>done by a committee under the auspices of ISAC, since that effort has<BR/>>essentially failed, principally through inaction. Rather, I think the<BR/>>Editorial Boards should empower a group to put such a document together. If<BR/>>such an effort works, it can serve as a model for other journals to adopt.<BR/>><BR/>>mrAnonymousnoreply@blogger.com