UCFlow

We have a replacement for the JDSU 355nm laser... ...

2010-02-24T16:40:58.290-08:00

We have a replacement for the JDSU 355nm laser... it is less expensive, and will last far longer than what you have been seeing on your machine. Please contact us if you are inetrested in this...
Eric@LaserInnovations.com

Who knows where to download XRumer 5.0 Palladium? ...

2009-11-21T07:59:55.312-08:00

Who knows where to download XRumer 5.0 Palladium?
Help, please. All recommend this program to effectively advertise on the Internet, this is the best program!

Thanks Ernie, I've included your reply above.

2009-11-13T07:40:30.629-08:00

Thanks Ernie, I've included your reply above.

FYI, batching stat and PDF output is pretty easy. ...

2009-11-09T08:35:25.261-08:00

FYI, batching stat and PDF output is pretty easy. Just load the files you want to process and select them in the analysis list. (The usual Windows SHIFT-click, CTRL-click, CTRL-A shortcuts work here.) When you have more than one analysis selected, the display is replaced by several buttons:

"Export statistics from selected" will produce a single file containing the displayed statistics from each analysis. Make sure you're showing the stats you want to display before you export. If you're not picky it's easy to just select all the plots and turn on all the stats.

"Print all sheets from selected" and "Print report sheets from selected" do what they say. If you want PDF output, choose a PDF printer driver. There's one on the program CD if you don't have one already. (Sorry, you didn't get that with the beta.)

Yes, we successfully managed to not talk about sci...

2009-10-10T07:54:30.882-07:00

Yes, we successfully managed to not talk about science for a single minute

So when is it coming?? :)

2009-10-08T12:25:47.784-07:00

So when is it coming??
:)

Yeah, we've been dealing with this for a while, bu...

2009-10-03T06:18:19.936-07:00

Yeah, we've been dealing with this for a while, but one week into the policy change, and our cancelations are waaaaaayyyyy down. Hopefully it lasts

AIM account is : flowlabhelp. Geez...

2009-09-27T20:19:02.048-07:00

AIM account is : flowlabhelp.

Geez...

It has to be just that time of year; we've just mo...

2009-09-27T19:19:01.948-07:00

It has to be just that time of year; we've just modified our policy to be remarkably similar (but 48 hours instead of 24).

we are in the process of buying a new flowcytomete...

2009-09-15T05:15:36.122-07:00

we are in the process of buying a new flowcytometer and have tested the BD Canto II and Beckman Coulter Galios. The latter comes out far better in all aspects.

Does anyone every tested the BD LSR fortessa and the Galios? Is the Galios superior to the LSR?

Love the live blogging!!

2009-05-21T10:43:21.944-07:00

Love the live blogging!!

send me an email at ucflow at gmail dot com and I'...

2009-05-11T06:52:51.796-07:00

send me an email at ucflow at gmail dot com and I'll send you the pdf of the galios and astrios specs.

Hi, can you tell where you got all these specs - a...

2009-05-06T04:58:18.535-07:00

Hi, can you tell where you got all these specs - and maybe link to them? I'm curious to find out more about these new offerings (badly overdue, and although I'm not a Coulter fan I do like having some more competition in this market), and I'm sure I'm not the only one.

Great info. We are in Day 2 of an aria sorp to and...

2009-03-19T08:38:43.198-07:00

Great info. We are in Day 2 of an aria sorp to and aria2 sorp info. Your pictures are almost a carbon copy of what I see right now. How about weekly strilization on the aria 2?. Different than the old way? bleach vs ethanol?

Interesting blog......

2009-02-25T14:43:50.767-08:00

Interesting blog......

yeah, it's pretty sweet. I'm glad we did it becau...

2009-02-18T09:38:34.620-08:00

yeah, it's pretty sweet. I'm glad we did it because our Aria I was really beat up.

Oh this sounds so NICE! Our AriaII is sitting rea...

2009-02-14T08:14:59.776-08:00

Oh this sounds so NICE! Our AriaII is sitting ready to be installed next week, and I've considered upgrading our Aria. Please keep posting on how the upgraded Aria acts long term!

(Maybe I can somehow scrape up $70k for an upgrade once we fall in love w/ the AriaII.... ;)

glad to see someone reads it : )

2009-02-09T18:56:56.899-08:00

glad to see someone reads it : )

Hi I just found out today that you guys keep a blo...

2009-02-06T13:45:30.838-08:00

Hi I just found out today that you guys keep a blog about Flow facility. It is nice.
I am excited to use Aria II soon.

Keep up the good work.

2008-11-10T17:39:23.493-08:00

Keep up the good work.

I know the request was for Mac software but someti...

2008-11-08T00:50:19.226-08:00

I know the request was for Mac software but sometimes we have to
swallow

From: David Novo (no...@fhs.csu.McMaster.CA)
Date: Thu Feb 26 1998 - 17:00:40 EST
• Next message: JoAnne Thomas: "FACSLoader Upgrade"
• Previous message: David McFarland: "MO drives and CellQuest
files"
• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
______________________________________...
If I am not mistaken, WinMDI comes with a little utility called
FCSread
that extracts file header information.

I know the request was for Mac software but sometimes we have to
swallow
our pride and admit we live in Bill's universe :-(

-Dave
______________________________________...
• Next message: JoAnne Thomas: "FACSLoader Upgrade"
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files"
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[ attachment ]
______________________________________...
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:35:13 EST

Wanted- FCS keyword read ut
From: Diether (diet...@amcell.com)
Date: Tue Feb 24 1998 - 07:57:50 EST
• Next message: Marty_Gied...@cc.chiron.com: "Annexin V
expression"
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markers"
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• Reply: Ray Hicks: "Re: Wanted- FCS keyword read ut"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
______________________________________...
Subject: Time:
13:54
OFFICE MEMO Wanted: FCS keyword read utility for Date:
2/24/98
I am looking for a utility program for the MacIntosh to read and
print
filenames
and FCS keywords like $BTIM and $ETIM.

Diether R.
AmCell Corp.
Ph.: USA-408-752-1200
FAX: USA-408-752-1212
Email: diet...@amcell.com or for binary attachments diethe...@usa.net
______________________________________...
• Next message: Marty_Gied...@cc.chiron.com: "Annexin V
expression"
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______________________________________...
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17:35:13 EST

Re: Wanted- FCS keyword read ut
From: David Novo (no...@fhs.csu.McMaster.CA)
Date: Thu Feb 26 1998 - 17:00:40 EST
• Next message: JoAnne Thomas: "FACSLoader Upgrade"
• Previous message: David McFarland: "MO drives and CellQuest
files"
• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
______________________________________...
If I am not mistaken, WinMDI comes with a little utility called
FCSread
that extracts file header information.

I know the request was for Mac software but sometimes we have to
swallow
our pride and admit we live in Bill's universe :-(

-Dave
______________________________________...
• Next message: JoAnne Thomas: "FACSLoader Upgrade"
• Previous message: David McFarland: "MO drives and CellQuest
files"
• In reply to: Ray Hicks: "Re: Wanted- FCS keyword read ut"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
______________________________________...
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:35:13 EST

Re: Wanted- FCS keyword read ut
From: Ray Hicks (rh...@cus.cam.ac.uk)
Date: Thu Feb 26 1998 - 05:10:28 EST
• Next message: Steve G. Hilliard: "For Palm Pilot users"
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[ attachment ]
______________________________________...
Hi Diether,

If you can't find one, try opening the files in microsoft Word
(you'll
probably need to use the "All Files" option),copy the text part to a
new
window and then get it to replace the delimiter with a paragraph
mark.
This
will give you a list that looks like this:

$TOT
5000
$P1R
1024
$P1B
16
$P2R
1024
$P2B
16
$P3R
1024
$P3B
16
$P4R
1024
$P4B [snip]

Rather than this:

\$TOT\5000\$P1R\1024\$P1B\16\$P2R\1024...
\1024\$P4B\

Purdue Cytometry Mailing List: SV: Thanks for the ...

2008-10-28T11:22:00.285-07:00

Purdue Cytometry Mailing List: SV: Thanks for the suggestions -

... cytometry/WEASELv2.html

http://www.wehi.edu.au/cytometry/WEASELv2.html

Winlist 3d from Verity House Software (www.vsh.com) Regards Tim

Timothy Bushnell, Ph.D. Director, CPBR ... Contemporary messages
sorted:

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Find Similar
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Re: Thanks for the suggestions - was rendering in 3D

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From: Adam Treister <...@treestar.com>
Date: Thu May 18 2006 - 20:20:18 EDT

On May 12, 2006, at 10:02 AM, Bushnell, Timothy wrote:

> Thanks to everyone who suggested possible software to view data in
> 3D. I’ll be trying several different platforms to see which works
> best for our applications.
>

> The suggested platforms include (in alphabetical order):
>

> Coulters CXP software for the FC500
>
> Rflowcyt
>

> Weasel: http://www.wehi.edu.au/cytometry/WEASELv2.html
>

> Winlist 3d from Verity House Software (www.vsh.com)
>
> Regards
>
> TimTim,

With all due respect to these solutions, you shouldn't think that

Mario could sleep at night

if anyone could perform high dimensional
analysis better than FlowJo.

[Disclaimer: Yes, I live off FlowJo
sales, and that's a blatantly commercial statement, but it gets
technical from here on.]

We've played with spatial 3D plots a lot, both our own prototypes
and
others, and they just don't do a very good job of discriminating
populations. And its impossible to coherently describe the
populations you can see.

I like the slice-and-dice approach that
you get by making a flipbook on X vs. Y in slices along the Z axis
(it shows up as a Quicktime movie), but that too is very difficult
to
use in a way that's better than two 2D graphs connected by a gate.
If you really want to increase your dimensionality,

we're just adding a new "Polyvariate Plot" to the Mac version of FlowJo for next
week's

ISAC.

The idea was taken from RFlowCyt. We've added interface
refinements to make it more interactive, but like any good R tool,
it'll astound and confuse you.
http://www.flowjo.com/v8/html/polyvarplot.html

The Polyvariate Plot can model transformations in any number of
dimensions. So it will produce a 3D plot, or as many dimensions as
you want (Shown below in 5D). And it projects these transformations
onto a graph window, so you can gate on them.

We're still trying to figure out the applications for this
visualization, but if you're looking for another dimension as a way
to differentiate populations, we think this is potentially much more
powerful than conventional spatial projections. This is explained
in the poster P178 at ISAC next week, or at the web page above.

Be forewarned: This is the opposite end of the sizzle/steak
spectrum. Most people use 3D graphs to make their PowerPoints look
spiffy. These graphs are absolutely impossible to explain in a
presentation.

Adam

-----------------------

A 3D plot:

A 5D plot:

Received on Fri May 19 17:58:00 2006
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Timothy: "Thanks for the suggestions - was
rendering in 3D"

Next in thread: Jerry Spangrude: "Making tandem conjugates"
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If anyone is interested in their software to funct...

2008-10-25T22:07:39.371-07:00

If anyone is interested in their software to function properly please
contact Kanecki Associates Inc.

In November of 2007 Kanecki Associates Inc developed the FCS Cytopro
to resolve the Memory issues with the Diva Software after a request
from one person at BD. 10 Million Events By 40 Parameters with all
128
Permeations.

As of today’s posts on the Purdue Cytometry Mail List the issues
still
exist.

Since are not allowed on the Purdue Cytometry Mail List and have ISAC
Congress Executives blocking us from the market we would like to make
BD and all other Corporations, Hospitals, Universities, etc. aware
that we can LICENSE A PATCH that will resolve the issues in BD's DIVA
software.

We are willing to work with BD and are unaware if they knew of the
one
person’s prior request or even what was done with the software once
the one person from BD received it. We will send a letter to BD's
corporate office to notify them we are willing to work with the
corporation if they wish. We did develop the code and are willing to
work directly with BD if they wish to compile it to the troubled
software to end all the issues.

There is no need for Purdue University Cytometry Laboratories
Executives to keep up with suspected exclusion of those who can help.
They need to resolve the issues in the system with the ISAC Congress
Executives in the Data Standards Committee and work with other
corporations even if the won't benefit financially personally.

If anyone is interested in their software to function properly please
contact Kanecki Associates Inc.

Http://www.kanecki.com
C...@Kanecki2.com
Mi...@kanecki.com

Mitchell Haynes
Vp.
Kanecki Associates Inc
281-444-1619

Diva 6.1.1 user issues
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From: Wayne Turnbull wayne.turnb...@kcl.ac.uk
Date: Tue Oct 14 2008 - 09:32:19 EDT

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT
Received on Tue Oct 14 11:58:00 2008
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RE: Diva 6.1.1 user issues
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From: Nilles, Tricia L.. tnil...@jhsph.edu
Date: Wed Oct 15 2008 - 09:43:57 EDT

Wayne,

Yes, we also have experienced a similar issue. After running CS&T,
we
cannot make any
changes (ie renaming specimens, experiments) we first have to exit
and
relaunch the
software. Logging out does not work. I have discussed this with our
local service rep
and also one of the software specialists who came in for local
training and demos, and no
one has offered a 'fix'.

Tricia L Nilles
Research Specialist
Flow Cytometry Laboratory
Johns Hopkins Bloomberg School of Public Health
Phone: 410-502-9290
Fax: 410-614-2503
-

-----Original Message-----
From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
Sent: Tuesday, October 14, 2008 9:32 AM
To: cyto-inbox
Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT
Received on Wed Oct 15 12:38:00 2008
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Cytometer anyone? Circa 1991"
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Re: Diva 6.1.1 user issues
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From: Berend b.hooibr...@amc.uva.nl
Date: Thu Oct 16 2008 - 03:58:54 EDT

We have seen similar problems with 6.1.1 and CST module. I am not
able to run the cst when I started the diva software in a Windows
(normal)User account. It locks-up immediately. So, we first log in
as
administrator in windows xp, do the cst run, quit diva after this qc-
check and log in as (limited) user. Thereafter we startup diva again
and everything works fine, except the cst and configuration modules.
Our service engineer (BD) couldn't fix it so far. Still I prefer the
let everybody work as limited user with the LSR, to keep systems
clean from al kind of ?%^&b 9b b " people want to install.
Berend

On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote:

> Wayne,

> Yes, we also have experienced a similar issue. After running CS&T,
> we cannot make any
> changes (ie renaming specimens, experiments) we first have to exit
> and relaunch the
> software. Logging out does not work. I have discussed this with
> our local service rep
> and also one of the software specialists who came in for local
> training and demos, and no
> one has offered a 'fix'.

> Tricia L Nilles
> Research Specialist
> Flow Cytometry Laboratory
> Johns Hopkins Bloomberg School of Public Health
> Phone: 410-502-9290
> Fax: 410-614-2503

> -----Original Message-----
> From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
> Sent: Tuesday, October 14, 2008 9:32 AM
> To: cyto-inbox
> Subject: Diva 6.1.1 user issues

> Afternoon flowers,

> Having recently moved to Diva 6.1.1, we're experiencing some software
> lock-up problems, usually after closing down CS&T, that require a
> full re-install, which is becoming tiresome, and I wondered if any
> others had experienced similar problems?

> Wayne

> Wayne Turnbull
> Flow Cytometry Services
> Department of Immunobiology
> GKT Guy's Hospital
> London SE1 9RT

Berend Hooibrink
Flow cytometry core facility
Dept.. Cellbiology & Histology room L3-115
AMC
Meibergdreef 15
1105AZ Amsterdam
Netherlands
Tel.: 31(0)20-5666804
Fax: 31(0)20-6974156
Received on Thu Oct 16 15:38:00 2008
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RE: Diva 6.1.1 user issues
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From: Ted Hibbs Ted_Hi...@bonfils.org
Date: Thu Oct 16 2008 - 22:27:10 EDT

Berend,

We had a similar issue when we first got our BD CantoII with Diva
6.1.1. It
is related to the permissions on the folders on the C:\ drive, though
I donb t
remember exactly where. Once I changed the permissions on the
folders, it is
working well.

Ted

From: Berend [mailto:b.hooibr...@amc.uva.nl]
Sent: Thursday, October 16, 2008 1:59 AM
To: Cytometry Mailing List
Subject: Re: Diva 6.1.1 user issues

We have seen similar problems with 6.1.1 and CST module. I am not
able
to run
the cst when I started the diva software in a Windows (normal)User
account.
It locks-up immediately. So, we first log in as administrator in
windows xp,
do the cst run, quit diva after this qc-check and log in as (limited)
user.
Thereafter we startup diva again and everything works fine, except
the
cst
and configuration modules. Our service engineer (BD) couldn't fix it
so far.
Still I prefer the let everybody work as limited user with the LSR,
to
keep
systems clean from al kind of ?%^&b 9b b " people want to install.

Berend

On Oct 15, 2008, at 3:43 PM, Nilles, Tricia L.. wrote:

Wayne,

Yes, we also have experienced a similar issue. After running CS&T, we
cannot
make any

changes (ie renaming specimens, experiments) we first have to exit
and
relaunch the

software. Logging out does not work. I have discussed this with our
local
service rep

and also one of the software specialists who came in for local
training and
demos, and no

one has offered a 'fix'.

Tricia L Nilles

Research Specialist

Flow Cytometry Laboratory

Johns Hopkins Bloomberg School of Public Health

Phone: 410-502-9290

Fax: 410-614-2503

-----Original Message-----

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]

Sent: Tuesday, October 14, 2008 9:32 AM

To: cyto-inbox

Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software

lock-up problems, usually after closing down CS&T, that require a

full re-install, which is becoming tiresome, and I wondered if any

others had experienced similar problems?

Wayne

Wayne Turnbull

Flow Cytometry Services

Department of Immunobiology

GKT Guy's Hospital

London SE1 9RT

Berend Hooibrink

Flow cytometry core facility

Dept.. Cellbiology & Histology room L3-115

AMC

Meibergdreef 15

1105AZ Amsterdam

Netherlands

Tel.: 31(0)20-5666804

Fax: 31(0)20-6974156

Confidentiality Notice: This e-mail message, including any
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disclosure or distribution is prohibited. If you are not the
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recipient, please contact the sender by reply e-mail and destroy all
copies of the original message.
Received on Fri Oct 17 13:18:00 2008
This message: [ Message body ]
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Cytometry"
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free
antibodies"
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RE: Diva 6.1.1 user issues
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From: Linkes, Sean Patrick sean.lin...@utoledo.edu
Date: Tue Oct 14 2008 - 16:11:56 EDT

No problems here. Our CS&T works wonderfully. Sometimes the setup
doesn't work right even with a freshly prepared tube of beads (with
the BD Field Service guy leaving one day prior having aligned the
lasers). Sean

Sean Linkes MS
Research Associate
Flow Cytometry Core Manager
The University of Toledo HSC
(419) 383 3402 http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html

________________________________

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
Sent: Tue 10/14/2008 9:32 AM
To: cyto-inbox
Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT
Received on Wed Oct 15 14:18:00 2008
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RE: Diva 6.1.1 user issues
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From: Roberts, Daniel Daniel.Robe...@covance.com
Date: Wed Oct 15 2008 - 18:53:42 EDT

I've been using 6.1.1 since May - no problems here. I'd question
your
Ram,
but if you bought the computer as part of the package - you should be
OK. I
know that sometimes leaving the beads on there can cause issues - but
If
memory serves (as opposed to habit) the CST software prompts/makes
you
take
the beads off. Other than that, I can't think of why it wouldn't
reconnect
to Diva.

Dan
Technical Resource Specialist
Covance Labs, NA
Vienna, VA 20850

-----Original Message-----
From: Linkes, Sean Patrick
To: cyto-inbox
Sent: 10/14/2008 3:11 PM
Subject: RE: Diva 6.1.1 user issues

No problems here. Our CS&T works wonderfully. Sometimes the setup
doesn't work right even with a freshly prepared tube of beads (with
the
BD Field Service guy leaving one day prior having aligned the
lasers).
Sean
Sean Linkes MS
Research Associate
Flow Cytometry Core Manager
The University of Toledo HSC
(419) 383 3402
http://hsc.utoledo.edu/depts/micro/UT_Flow_Cytometry.html

_____

From: Wayne Turnbull [mailto:wayne.turnb...@kcl.ac.uk]
Sent: Tue 10/14/2008 9:32 AM
To: cyto-inbox
Subject: Diva 6.1.1 user issues

Afternoon flowers,

Having recently moved to Diva 6.1.1, we're experiencing some software
lock-up problems, usually after closing down CS&T, that require a
full re-install, which is becoming tiresome, and I wondered if any
others had experienced similar problems?

Wayne

Wayne Turnbull
Flow Cytometry Services
Department of Immunobiology
GKT Guy's Hospital
London SE1 9RT

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If anyone is interested in their software to function properly please
contact Kanecki Associates Inc.

Http://www.kanecki.com
Ceo@kanecki.com
Mitch@kanecki.com

Mitchell Haynes
Vp.
Kanecki Associates Inc
281-444-1619

Thank you

Purdue University Cytometry Labatories & Mail List...

2008-10-14T22:12:11.434-07:00

Purdue University Cytometry Labatories & Mail List
RE: Bad Flow Data & reviewing -- What can we do? From: Claudio Vallan
(claudio.val...@dkf7.unibe.ch) Date: Thu Oct 25 2001 - 02:39:08 EST •
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Shapiro: "Re:Virus Sorting" • Previous message: Maciej Simm: "Re: Bad
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[ attachment ] ________________________________________

Maybe

it is not a bad idea to make post print reviews, white papers,
instructions to authors etc. etc.
But I think al of this will be mainly for

our own use. "Normal" scientist will probably never know that such
stuff even exists.

I think that the only way to make scientist aware of the problems
thay
may encounter when generating cytometric
datas

is to infiltrate their meetings and conferences and have the guts to
stand up

and tell them that what they are showing on that slide is just
bullshit.

If that happens a few time they will certainly have a closer look at
what they are doing.

Though, I do not think they will take us very seriously if we just
tell them that they have mislabelled the axes.

Claudio Vallan ===================================================
Claudio Vallan PhD Phone Lab: 031 / 632 88 76 FACS-LAB DKF Phone
Office: 031 / 632 99 68 University of Bern E-Mail:
val...@dkf7.unibe.ch c/o Institute of Pathology Murtenstrasse 31
Insel
hosptial area only: 3010
Bern Beeper: 181 67 59
\
On Aug 20, 2004, at 2:25 PM, J. Paul Robinson wrote:

RE: ISAC - MultiColor Flow CytometryThis message: ...

2008-10-14T21:36:29.594-07:00

RE: ISAC - MultiColor Flow Cytometry

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From: Marty Bigos mbigos@gladstone.ucsf.edu

Date: Tue Apr 27 2004 - 19:21:42 EST

Just to give credit where it is due:

The presentations at this workshop were (in order of appearance):

Marty Bigos - Gladstone Institutes - Basic Concepts for Compensation

Mario Roederer - NIH - The Hard, the Bad, and the Ugly

Nicole Baumgarth - UC Davis - The "How-to Guide" to Compensation for

Multicolor Cytometry

Panel - the above 3 joined by Bill Hyun (UCSF) and David Parks (Stanford)

The workshop was organized by Treestar, Inc. and the Gladstone Flow

Core. Many companies contributed to making it happen (in alphabetical

order):

Amnis, BD Biosciences, Beckman-Coulter, Caltag, DakoCytomation,

DeNovo Software, FloCyte Associates, Miltenyi, and Southern BioTech.

Paul Robinson and Bartek Rajwa did the video post-production.

Marty

>The question has been asked about videoing ISAC

>workshops and tutorials. On CD8 which will be coming out

>at ISAC, we have a bonus. CD8 is a 2 cd set and the

>second CD is an outstanding tutorial that Mario and Marty

>Bigos and others put on in San Francisco last year. Its really

>good!

>

>We would like to do this for selected tutorials even at ISAC

>and we are developing a proposal for ISAC as we speak. It

>would help to know if this was something of interest.

>

>Regards

>paul robinson

>Purdue

>

>

>

>On 26 Apr 2004 at 11:03, Kroeger, Jodi wrote:

>

>> What are the chances that this tutorial, (and others of high

>>interest) could be

>> video taped and broadcasted over the internet for those

>>unfortunate souls unable

>> to attend? At least to those of us who are ISAC members?

>>

>> -----Original Message-----

>> From: Mario Roederer [mailto:roederer@drmr.com]

>> Sent: Thursday, April 22, 2004 11:48 PM

>> To: cyto-inbox

>> Subject: ISAC - MultiColor Flow Cytometry

>>

>>

>> For the second straight ISAC (officially making this a tradition),

>> I'll be running a Saturday tutorial on Multicolor Flow Cytometry.

>> This time, instead of being at the unhappy hour of 8 in the morning,

>> it will be at a much more civilized early afternoon time.

>>

>> This tutorial (aptly numbered: Tutorial "X") will be much more

>> pragmatic in nature than previous. We will have three talks that

>> will center on how to plan and implement a multicolor

>> immunophenotyping experiment, including instrument setup and

>> validation (Steve Perfetto), multicolor panel design and optimization

>> (myself), and bridging the gap to the clinic (Brent Wood). Assuming

>> that most of us are not in a full post-prandial siesta mode, this

>> should be a highly interactive session: please come with your

>> questions, data, artefacts, and complaints in hand!

>>

>> If you are jumping on the 7, 10, or 15-color bandwagon, then this

>> tutorial is for you! If you've never done more than 2-color

>> staining, then enjoy the wonderful city of Montpellier instead :)

>>

>> If you have any suggestions for specific topics, or any general

>> questions, please send them to me in advance and we will try to

>> address them.

>>

>> Regards,

>>

>> mr

>> ######################################################################

>> This transmission may be confidential or protected from disclosure and

>> is only for review and use by the intended recipient. Access by

>> anyone else is unauthorized. Any unauthorized reader is hereby

>> notified that any review, use, dissemination, disclosure or copying of

>> this information, or any act or omission taken in reliance on it, is

>> prohibited and may be unlawful. If you received this transmission in

>> error, please notify the sender immediately. Thank you.

>>

>> ######################################################################

>>

>

>

>J.Paul Robinson, PhD PH:(765)4940757

>Professor of Immunopharmacology

>Professor of Biomedical Engineering

>Purdue University FAX:(765)4940517

>EMAIL:jpr@flowcyt.cyto.purdue.edu

>WEB: http://www.cyto.purdue.edu

>

>Have you seen our new HCS webpage?

>http://www.cyto.purdue.edu/hcs

Received on Wed Apr 28 16:56:53 2004

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